Maleszka R, Clark-Walker G D
Molecular & Population Genetics Group, Research School of Biological Sciences, Australian National University, Canberra.
Yeast. 1993 Jan;9(1):53-8. doi: 10.1002/yea.320090107.
By employing pulsed-field gel electrophoresis we have determined the size of the rDNA cluster in wild-type yeast strains representing genera of Candida, Kluyveromyces, Pachysolen, Schizosaccharomyces and Torulaspora. Although the genome size of the examined species is similar (12.3-13.9 Mb), at least a four-fold variation has been observed between the lowest amount of rDNA repeats in P. tannophilus (28) and the highest in C. glabrata and S. poombe (> 115). In two species the rDNA cluster is represented by two loci, residing either in one (S. pombe) or two chromosomes (C. glabrata).
通过使用脉冲场凝胶电泳，我们已经确定了代表念珠菌属、克鲁维酵母属、管囊酵母属、裂殖酵母属和德巴利酵母属的野生型酵母菌株中rDNA簇的大小。尽管所检测物种的基因组大小相似（12.3 - 13.9 Mb），但在嗜单宁管囊酵母中rDNA重复序列的最低数量（28个）与光滑念珠菌和粟酒裂殖酵母中的最高数量（> 115个）之间，观察到了至少四倍的差异。在两个物种中，rDNA簇由两个位点代表，它们位于一条染色体（粟酒裂殖酵母）或两条染色体（光滑念珠菌）上。
