人成骨样骨肉瘤细胞中的芳香化酶活性。
Aromatase activity in human osteoblast-like osteosarcoma cell.
作者信息
Tanaka S, Haji M, Nishi Y, Yanase T, Takayanagi R, Nawata H
机构信息
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
出版信息
Calcif Tissue Int. 1993 Feb;52(2):107-9. doi: 10.1007/BF00308318.
Aromatase activity was studied in cultured human osteosarcoma cell (HOS). HOS was incubated from 12 to 72 hours with 10(-10) M-10(-5) M dexamethasone. Aromatase activity was determined by measuring [3H]H2O released upon the conversion of [1 beta-3H]androstenedione to estrone. HOS showed aromatase activity, and apparent km for [1 beta-3H]androstenedione was 4.46 +/- 0.98 nm (mean +/- SD). The aromatase activity was significantly increased by 10(-9) M-10(-5) M dexamethasone in a dose-dependent manner. Dexamethasone increased Vmax of aromatase activity but did not change its km value. These results suggest that osteoblastic cells have aromatase activity which is regulated by glucocorticoid, and directly convert androgen to estrogen in itself.
在培养的人骨肉瘤细胞（HOS）中研究了芳香化酶活性。将HOS与10（-10）M - 10（-5）M地塞米松孵育12至72小时。通过测量[1β-3H]雄烯二酮转化为雌酮时释放的[3H]H2O来测定芳香化酶活性。HOS显示出芳香化酶活性，[1β-3H]雄烯二酮的表观km为4.46±0.98纳米（平均值±标准差）。10（-9）M - 10（-5）M地塞米松以剂量依赖性方式显著增加芳香化酶活性。地塞米松增加了芳香化酶活性的Vmax，但未改变其km值。这些结果表明，成骨细胞具有受糖皮质激素调节的芳香化酶活性，并能自身将雄激素直接转化为雌激素。
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