Caturegli P, Kuppers R C, Mariotti S, Burek C L, Pinchera A, Ladenson P W, Rose N R
Department of Molecular Microbiology and Immunology, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD 21205.
Clin Exp Immunol. 1994 Dec;98(3):464-9. doi: 10.1111/j.1365-2249.1994.tb05514.x.
The IgG subclass distribution of thyroglobulin antibodies (TgAb) has been studied in Hashimoto and Graves' patients by several investigators with conflicting results, in part explainable by methodological problems. We have recently developed a quantitative ELISA to measure in absolute terms the serum concentration of TgAb subclasses. The aim of the present study was to apply this method in a large series of patients with autoimmune as well as, for the first time, non-autoimmune thyroid diseases. We examined 28 patients with Hashimoto's thyroiditis, 30 with Graves' disease, 21 with thyroid carcinoma and 18 with non-toxic goitre, all selected for the presence of TgAbs. The results indicated that TgAbs in thyroid diseases were not restricted to any particular isotype, but comprised all four IgG subclasses. IgG1 was represented similarly in the four groups. The same was true for IgG3, even though its contribution to the total antibody content was very small. IgG4 was the dominant subclass in patients with Graves' disease, thyroid carcinoma and non-toxic goitre, probably reflecting a prolonged antigenic challenge. In Hashimoto's thyroiditis IgG2 was dominant, possibly because T helper lymphocytes infiltrating the thyroid are typically Th1 type.
几位研究者对桥本氏病和格雷夫斯病患者甲状腺球蛋白抗体（TgAb）的IgG亚类分布进行了研究，但结果相互矛盾，部分原因可归因于方法学问题。我们最近开发了一种定量酶联免疫吸附测定法（ELISA），以绝对数值测量TgAb亚类的血清浓度。本研究的目的是将该方法应用于一大组自身免疫性以及首次应用于非自身免疫性甲状腺疾病患者。我们检查了28例桥本氏甲状腺炎患者、30例格雷夫斯病患者、21例甲状腺癌患者和18例非毒性甲状腺肿患者，所有患者均因存在TgAb而入选。结果表明，甲状腺疾病中的TgAb不限于任何特定的同种型，而是包括所有四种IgG亚类。IgG1在四组中的表现相似。IgG3也是如此，尽管其在总抗体含量中的占比非常小。IgG4是格雷夫斯病、甲状腺癌和非毒性甲状腺肿患者中的主要亚类，这可能反映了抗原刺激时间延长。在桥本氏甲状腺炎中，IgG2占主导地位，可能是因为浸润甲状腺的辅助性T淋巴细胞通常是Th1型。
