Yamamoto T, Moriwaki Y, Suda M, Agbedana E O, Takahashi S, Nasako Y, Higashino K, Nakamo T, Hiroishi K
Third Department of Internal Medicine, Hyogo College of Medicine, Japan.
Int J Clin Pharmacol Ther Toxicol. 1993 Jan;31(1):35-9.
We administered xylitol intravenously to normal subjects to investigate the mechanism of xylitol-induced increase in the purine degradation in humans. Xylitol increased the plasma concentrations of hypoxanthine, xanthine and uric acid but decreased the blood concentration of pyruvic acid. The erythrocyte concentrations of IMP, AMP, ADP, glyceraldehyde 3-phosphate and fructose 1,6-diphosphate as well as the urinary excretion of hypoxanthine and xanthine were increased, while the erythrocyte concentration of ATP was decreased. In addition, the in vitro incubation studies using erythrocytes demonstrated that both xylitol-induced purine degradation and xylitol-induced inhibition of the conversion of glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate were protected by pyruvic acid. These results indicate that xylitol-induced impairment of glycolysis in erythrocytes contributes to the observed xylitol-induced increase in the purine degradation in the body.
我们对正常受试者静脉注射木糖醇，以研究木糖醇诱导人体嘌呤降解增加的机制。木糖醇增加了次黄嘌呤、黄嘌呤和尿酸的血浆浓度，但降低了丙酮酸的血液浓度。红细胞中肌苷一磷酸（IMP）、腺苷一磷酸（AMP）、二磷酸腺苷（ADP）、3-磷酸甘油醛和1,6-二磷酸果糖的浓度以及次黄嘌呤和黄嘌呤的尿排泄量增加，而红细胞中三磷酸腺苷（ATP）的浓度降低。此外，使用红细胞进行的体外孵育研究表明，丙酮酸可保护木糖醇诱导的嘌呤降解以及木糖醇诱导的3-磷酸甘油醛向1,3-二磷酸甘油酸转化的抑制作用。这些结果表明，木糖醇诱导的红细胞糖酵解损伤导致了体内观察到的木糖醇诱导的嘌呤降解增加。
