Loubeyre C, Desnottes J F, Moreau N
Centre National de la Recherche Scientifique, CERCOA, Thiais, France.
J Antimicrob Chemother. 1993 Jan;31(1):37-45. doi: 10.1093/jac/31.1.37.
The effect of sub-inhibitory concentrations of antibacterials, including quinolones, on the surface properties of a uropathogenic strain of Escherichia coli was examined. The effect on the charge and hydrophobicity of the cell surface was assessed by means of partition between two aqueous phases, polyethylene glycol and dextran. Antibiotics at 1/8 x MIC inhibited adhesion to uroepithelial cells, and induced an increase in bacterial charge and hydrophobicity. Inhibition of adhesion correlated with increased charge, but not with hydrophobicity. The influence of magnesium on the inhibition of adhesion by sub-MICs of pefloxacin was also investigated. Loss of the anti-adhesive property of pefloxacin was observed with increasing magnesium concentrations, suggesting that quinolones should be free from magnesium to induce an inhibition of adhesion. Examination by electron microscopy showed a disappearance of fimbriae following treatment of E. coli cells with 1/8 x MIC of pefloxacin.
研究了亚抑菌浓度的抗菌药物（包括喹诺酮类）对尿路致病性大肠杆菌菌株表面特性的影响。通过聚乙二醇和葡聚糖这两个水相之间的分配来评估对细胞表面电荷和疏水性的影响。1/8倍最低抑菌浓度（MIC）的抗生素可抑制对尿道上皮细胞的黏附，并导致细菌电荷和疏水性增加。黏附抑制与电荷增加相关，但与疏水性无关。还研究了镁对培氟沙星亚MIC抑制黏附的影响。随着镁浓度的增加，观察到培氟沙星抗黏附特性丧失，这表明喹诺酮类药物应不含镁才能诱导黏附抑制。电子显微镜检查显示，用1/8倍MIC的培氟沙星处理大肠杆菌细胞后菌毛消失。
