采用十八烷基硅烷化多孔玻璃高效液相色谱法测定生药中的酸性皂苷。
Determination of acidic saponins in crude drugs by high-performance liquid chromatography on octadecylsilyl porous glass.
作者信息
Kanazawa H, Nagata Y, Matsushima Y, Tomoda M, Takai N
机构信息
Kyoritsu College of Pharmacy, Tokyo, Japan.
出版信息
J Chromatogr. 1993 Feb 5;630(1-2):408-14. doi: 10.1016/0021-9673(93)80479-r.
PMID:8445006
Abstract
High-performance liquid chromatographic (HPLC) analysis on octadecylsilyl porous glass was investigated for acidic saponins in ginseng, bupleurum root and senega. The acidic saponins, malonyl-ginsenosides, malonyl-saikosaponins and senegins, as well as neutral saponins in the crude drugs were separated rapidly by HPLC on this column with aqueous acetonitrile containing KH2PO4 as the mobile phase at room temperature.
摘要
研究了在十八烷基硅烷化多孔玻璃上进行高效液相色谱（HPLC）分析人参、柴胡和远志中的酸性皂苷。以含KH2PO4的乙腈水溶液为流动相，在室温下，通过该柱上的HPLC可快速分离出药材中的酸性皂苷、丙二酰人参皂苷、丙二酰柴胡皂苷和远志皂苷以及中性皂苷。
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