Fuzzati N, Gabetta B, Jayakar K, Pace R, Peterlongo F
Indena SpA, Laboratori Ricerca e Sviluppo, Settala (Milan), Italy.
J Chromatogr A. 1999 Aug 27;854(1-2):69-79. doi: 10.1016/s0021-9673(99)00463-x.
A high-performance liquid chromatographic method was developed for electrospray mass spectrometric analysis of ginsenosides in Panax ginseng roots. The analyses were performed on a reversed-phase C18 column using a binary eluent (aqueous 8 mM NH4OAc, buffered to pH 7 with NH4OH-acetonitrile) under gradient conditions. Twenty-five ginsenosides could be separated and detected. The mass spectra obtained provided information on their molecular masses. A MS-MS experiment was undertaken in order to determine the sugar unit sequences and the aglycone moieties.
建立了一种高效液相色谱法，用于人参根中人参皂苷的电喷雾质谱分析。分析在反相C18柱上进行，使用二元洗脱液（8 mM醋酸铵水溶液，用氢氧化铵 - 乙腈缓冲至pH 7）在梯度条件下进行。可以分离并检测到25种人参皂苷。获得的质谱提供了它们分子量的信息。进行了串联质谱实验以确定糖单元序列和苷元部分。
