Ohmori S, Harada K, Miura H
Department of Hygiene, Kumamoto University School of Medicine.
Sangyo Igaku. 1993 Jan;35(1):25-34. doi: 10.1539/joh1959.35.25.
An experimental study on Japanese White (JW) rabbits was conducted to clarify the sex difference in FEP level. Male and female rabbits (n = 14 of each sex) were used. The animals of the same sex were divided into 3 groups; i.e., control group (5% glucose solution only, n = 4), low lead dose group (Pb 0.4 mg/kg.BW, n = 5) and high lead dose group (Pb 2 mg/kg.BW, n = 5). Lead was injected intravenously twice a week for 5 wk. The following parameters were determined once a week for 5 wk: blood lead (Pb-B), FEP, Ht, Hb, erythrocyte ALA-D activity, erythrocyte pyrimidine 5'-nucleotidase (P5N), urinary coproporphyrin (CP-U), urinary delta-aminolevulinic acid (ALA-U), iron in serum (Fe-S), and serum GOT and GTP. Average levels of FEP in female rabbits were higher than those in males between the 1st and 3rd week after the lead injection in the low lead dose groups, and in the final week in the high lead dose groups. In the periods without lead injection, the average levels of FEP in the female groups were not significantly higher than the corresponding levels in the male groups in every week except in the first week in the control. However, the mean of FEP levels in all female rabbits without Pb treatment was higher than that in male rabbits (t-test). By the analysis of variance for the gains of FEP from the initial value, only the low lead dose group showed a significant sex difference (female > male); that is, the female group tended to increase when compared with the male group. Furthermore, the week when FEP began to increase in the female groups was earlier than that in the male groups in the low lead dose group. In the high lead dose group, both sexes reacted to the lead exposure from the same early week. As for the parameters of anemia, the average levels of Ht and Hb tended to be lower in females than in males, but Fe-S levels were not affected by lead in both sexes and no consistent sex difference could be observed. By lead exposure, ALA-D and P5N were inhibited, and ALA-U was increased, but these parameters showed no evident sex difference. The average levels of CP-U tended to be higher in females than in males in the administration of low lead dose and to be inversely higher in males than in females in the administration of high lead dose.(ABSTRACT TRUNCATED AT 400 WORDS)
为了阐明游离原卟啉（FEP）水平的性别差异，对日本白兔进行了一项实验研究。使用了雄性和雌性兔子（每种性别各14只）。将同性别的动物分为3组；即对照组（仅5%葡萄糖溶液，n = 4）、低铅剂量组（Pb 0.4 mg/kg体重，n = 5）和高铅剂量组（Pb 2 mg/kg体重，n = 5）。每周静脉注射铅2次，共5周。在5周内每周测定一次以下参数：血铅（Pb-B）、FEP、血细胞比容（Ht）、血红蛋白（Hb）、红细胞δ-氨基-γ-酮戊酸脱水酶（ALA-D）活性、红细胞嘧啶5'-核苷酸酶（P5N）、尿粪卟啉（CP-U）、尿δ-氨基-γ-酮戊酸（ALA-U）、血清铁（Fe-S）以及血清谷草转氨酶（GOT）和谷丙转氨酶（GTP）。在低铅剂量组中，铅注射后第1至3周以及高铅剂量组的最后一周，雌性兔子的FEP平均水平高于雄性兔子。在无铅注射期间，除对照组的第一周外，雌性组的FEP平均水平在每周均未显著高于雄性组相应水平。然而，所有未接受铅处理的雌性兔子的FEP水平均值高于雄性兔子（t检验）。通过对FEP相对于初始值的增加量进行方差分析，只有低铅剂量组显示出显著的性别差异（雌性>雄性）；也就是说，与雄性组相比，雌性组有升高的趋势。此外，在低铅剂量组中，雌性组FEP开始升高的周数早于雄性组。在高铅剂量组中，两性从同一早期周开始对铅暴露产生反应。至于贫血参数，雌性的Ht和Hb平均水平往往低于雄性，但Fe-S水平在两性中均不受铅的影响，且未观察到一致的性别差异。通过铅暴露，ALA-D和P5N受到抑制，ALA-U升高，但这些参数未显示出明显的性别差异。在低铅剂量给药时，CP-U的平均水平雌性往往高于雄性，而在高铅剂量给药时，雄性则反而高于雌性。（摘要截断于400字）
