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通过扩增16S rRNA序列快速检测和鉴定鸟分枝杆菌

Rapid detection and identification of Mycobacterium avium by amplification of 16S rRNA sequences.

作者信息

van der Giessen J W, Eger A, Haagsma J, van der Zeijst B A

机构信息

Department of Bacteriology, School of Veterinary Medicine, University of Utrecht, The Netherlands.

出版信息

J Clin Microbiol. 1993 Sep;31(9):2509-12. doi: 10.1128/jcm.31.9.2509-2512.1993.

DOI:10.1128/jcm.31.9.2509-2512.1993
PMID:7691877
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC265788/
Abstract

An assay that is based on the amplification of 16S rRNA sequences and that was initially developed to detect Mycobacterium paratuberculosis in cattle was used to test 20 serotypes of the Mycobacterium avium complex (MAC) and atypical mycobacterial species not belonging to MAC. Only serotypes 1 to 6 and 8 to 11, designated M. avium, were detected by the assay, indicating that it can be used for the rapid detection and identification of M. avium. The results of the assay for clinical samples from animals suspected of having mycobacterial infections indicated that it can also be used directly on clinical samples.

摘要

一种基于16S rRNA序列扩增且最初用于检测牛副结核分枝杆菌的检测方法，被用于检测鸟分枝杆菌复合群（MAC）的20个血清型以及不属于MAC的非典型分枝杆菌菌种。该检测方法仅检测到了血清型1至6以及8至11，即鸟分枝杆菌，这表明它可用于鸟分枝杆菌的快速检测和鉴定。对疑似患有分枝杆菌感染的动物临床样本的检测结果表明，该方法也可直接用于临床样本。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc8/265788/7a833a13e4d6/jcm00021-0277-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc8/265788/40032d153f59/jcm00021-0276-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc8/265788/9e30fc002ca0/jcm00021-0277-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc8/265788/7a833a13e4d6/jcm00021-0277-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc8/265788/40032d153f59/jcm00021-0276-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc8/265788/9e30fc002ca0/jcm00021-0277-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fc8/265788/7a833a13e4d6/jcm00021-0277-b.jpg

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本文引用的文献

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Genus-specific polymerase chain reaction for the mycobacterial dnaJ gene and species-specific oligonucleotide probes.用于分枝杆菌dnaJ基因的属特异性聚合酶链反应和种特异性寡核苷酸探针。
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Mycobacterial infections in animals.
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Relationship between Mycobacterium avium, Mycobacterium paratuberculosis and "wood pigeon mycobacteria". Determinations by DNA-DNA hybridization.鸟分枝杆菌、副结核分枝杆菌与“林鸽分枝杆菌”之间的关系。通过DNA-DNA杂交进行测定。
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