Vajpayee R B, Angra S K, Sandramouli S, Honavar S G, Chhabra V K
Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, Ansari Nagar, New Delhi.
Ann Ophthalmol. 1993 Feb;25(2):68-71.
In a prospective study, we evaluated the sensitivities of direct microscopy using potassium hydroxide 10% wet-mount preparations and culture for definitive diagnosis of keratomycosis in 171 clinically suspected fungal corneal ulcers. Although cultures were positive in 88 eyes (51.46%), the fungus could be demonstrated by KOH preparation in 94.3% (83 of 88) of the culture-proved cases and 93.6% (160 of 171) of the overall eyes. Direct microscopic examination of a KOH wet-mount preparation is a rapid, reliable, and superior technique of laboratory diagnosis. It is particularly important for the early initiation of antifungal treatment in mycotic keratitis.
在一项前瞻性研究中，我们评估了使用10%氢氧化钾湿片制备的直接显微镜检查和培养对171例临床疑似真菌性角膜溃疡进行角膜真菌病确诊诊断的敏感性。虽然88只眼（51.46%）培养呈阳性，但在培养证实的病例中，94.3%（88例中的83例）的真菌可通过氢氧化钾制剂显示，在所有眼中为93.6%（171例中的160例）。氢氧化钾湿片制备的直接显微镜检查是一种快速、可靠且优越的实验室诊断技术。它对于真菌性角膜炎抗真菌治疗的早期启动尤为重要。
