Katz J, Wals P, Golden S, Goldman J K, Bernardis L L
Horm Metab Res. 1977 Jan;9(1):59-63. doi: 10.1055/s-0028-1093584.
Hepatocytes were prepared from livers of rats with bilateral lesions of the ventromedial hypothalamic nucleus (VMN lesioned) and sham-operated control rats. The incorporation of tritium labeled water and 14C glucose and lactate into lipids was studied. Endogenous tritium incorporation into fatty acids in controls ranged from 7-20 muatoms 3H per 100 mg liver protein per hour, and in cells from VMN lesioned rats 25 to 80 muatoms per 100 mg/hour. In both types of cells addition of glucose had no effect on tritium incorporation, but the addition of lactate, pyruvate or alanine stimulated it 30-60%. There was little difference in the labeling of glycerol by the two groups. In cells from fasted refed control or lesioned rats, tritium incorporation was greatly increased and was equal in both groups. Incorporation of glucose carbon in VMN lesioned rats was three to ten times that of controls, but the contribution of glucose carbon to fatty acid synthesis was small.
从双侧腹内侧下丘脑核损伤（VMN损伤）大鼠和假手术对照大鼠的肝脏中制备肝细胞。研究了氚标记水、14C葡萄糖和乳酸掺入脂质的情况。对照组中内源性氚掺入脂肪酸的量为每小时每100毫克肝脏蛋白7 - 20微摩尔3H，而VMN损伤大鼠细胞中为每100毫克/小时25至80微摩尔。在两种类型的细胞中，添加葡萄糖对氚掺入没有影响，但添加乳酸、丙酮酸或丙氨酸可使其增加30 - 60%。两组对甘油的标记差异不大。在禁食后再喂食的对照或损伤大鼠的细胞中，氚掺入量大幅增加且两组相等。VMN损伤大鼠中葡萄糖碳的掺入量是对照组的三到十倍，但葡萄糖碳对脂肪酸合成的贡献较小。
