Osada J, Garcés C, Sastre J, Schaefer E J, Ordovás J M
Departmento de Fisiologia, Facultad de Medicina, Universidad de Valencia, Spain.
Biochim Biophys Acta. 1993 Mar 20;1172(3):340-2. doi: 10.1016/0167-4781(93)90227-5.
A clone containing the coding region for cynomolgus monkey (Macaca fascicularis) apolipoprotein A-II has been isolated from a cynomolgus genomic DNA library. The gene spans 1.4 kilobases (kb). The complete nucleic acid sequence of the apolipoprotein A-II gene has been determined, establishing that the gene is interrupted by three intervening sequences of 170, 273 and 394 bp, respectively. The open reading frame encodes a protein of 100 amino acids, and shows 94% sequence similarity with its human equivalent. Both apolipoproteins have identical signal peptide. A noticeable feature is the substitution of mature human Cys-6 for Ser. This change explains the existence of cynomolgus apolipoprotein A-II as a monomer and may have important consequences in the kinetics of this apolipoprotein.
已从食蟹猴基因组DNA文库中分离出一个包含食蟹猴（猕猴）载脂蛋白A-II编码区的克隆。该基因跨度为1.4千碱基（kb）。已确定载脂蛋白A-II基因的完整核酸序列，证实该基因被分别为170、273和394 bp的三个间隔序列所中断。开放阅读框编码一个由100个氨基酸组成的蛋白质，与人类相应蛋白质的序列相似性为94%。两种载脂蛋白具有相同的信号肽。一个显著特征是成熟的人类半胱氨酸-6被丝氨酸取代。这一变化解释了食蟹猴载脂蛋白A-II以单体形式存在的原因，并且可能对该载脂蛋白的动力学产生重要影响。
