Crossen P E, Drets M E, Arrighi F E, Johnston D A
Hum Genet. 1977 Mar 14;35(3):345-52. doi: 10.1007/BF00446625.
Lymphocytes from 20 notmal subjects (11 male and 9 female) were examined for the frequency and location of sister chromatid exchanges (SCE) by the BrdU--Giemsa method. The mean frequency of SCE was 6.37 with little significant variation. One subject had a high number of exchanges in chromosome 1 while the remainder showed a random distribution of exchanges between chromosomes. The frequency of exchanges generally increased with chromosome length. However, chromosome 1, 2 and the B group had more exchanges than expected while the E, F and G grous had less than expected. The distribution of exchanges in chromosomes 1, 2 and the B group was non-random with a concentration of exchanges below the centromere and to a lesser extent on the distal portion of the long arm. The majority of exchanges appeared to occur at the junction between the dark and light G bands. It is suggested that the concentration of exchanges may reflect differences in BrdU incorporation along the length of the chromosome.
采用BrdU - 吉姆萨法，对20名正常受试者（11名男性和9名女性）的淋巴细胞进行了姐妹染色单体交换（SCE）频率及位置的检测。SCE的平均频率为6.37，差异无统计学意义。1名受试者1号染色体上的交换次数较多，其余受试者的交换在各染色体间呈随机分布。交换频率一般随染色体长度增加而升高。然而，1号、2号染色体及B组染色体的交换次数多于预期，而E、F和G组染色体的交换次数少于预期。1号、2号染色体及B组染色体上的交换分布并非随机，着丝粒下方交换集中，长臂远端也有较少程度的集中。大多数交换似乎发生在明暗相间的G带交界处。提示交换的集中可能反映了沿染色体长度方向BrdU掺入的差异。
