van Raamsdonk M, de Soet J J, Jones C L, de Graaff J
Department of Oral Microbiology, Academic Centre for Dentistry Amsterdam, The Netherlands.
Caries Res. 1997;31(1):35-40. doi: 10.1159/000262371.
Passive immunization has been suggested as a method to prevent colonization of teeth by mutans streptococci. However, the mechanism of action of antibodies, both polyclonal and monoclonal, is not clear. In this study we investigated the effect of polyclonal antibodies (pAbs) and a monoclonal antibody (OMVU10) on the chain length and growth of Streptococcus sobrinus. During growth in broth S. sobrinus formed significantly longer chains in the presence of pAbs in comparison to pre-immune serum (p < 0.01), but pAbs did not influence the growth rate of S. sobrinus in broth. OMVU10 did not influence the growth rate nor the chain length. In order to study the effect of the antibodies on adhesion and growth on a surface, S. sobrinus was grown on hydroxyapatite discs in the presence of two other bacteria, Streptococcus oralis and Actinomyces viscosus. No effect of the antibodies was found on the total cultivable count of the three bacteria after growth on hydroxyapatite discs. However, the morphology of the S. sobrinus microcolonies was different after growth in the presence of both pAbs and OMVU10. The colonies were less dense and chains of S. sobrinus could be seen using a confocal laser microscope. After growth in the presence of the control antibodies, the colonies were dense and no long chains could be observed. It was concluded that pAbs influenced the chain length, in broth and on hydroxyapatite discs, and the colony morphology of S. sobrinus on hydroxyapatite discs, whereas OMVU10 influenced the colony morphology of S. sobrinus on hydroxyapatite discs.
被动免疫已被提议作为一种预防变形链球菌在牙齿上定植的方法。然而，多克隆抗体和单克隆抗体的作用机制尚不清楚。在本研究中，我们调查了多克隆抗体（pAbs）和一种单克隆抗体（OMVU10）对嗜热栖热菌链长度和生长的影响。在肉汤中生长时，与免疫前血清相比，嗜热栖热菌在存在pAbs的情况下形成的链明显更长（p < 0.01），但pAbs不影响嗜热栖热菌在肉汤中的生长速率。OMVU10既不影响生长速率也不影响链长度。为了研究抗体对在表面上黏附和生长的影响，嗜热栖热菌在存在另外两种细菌——口腔链球菌和黏性放线菌的情况下在羟基磷灰石圆盘上生长。在羟基磷灰石圆盘上生长后，未发现抗体对这三种细菌的总可培养计数有影响。然而，在同时存在pAbs和OMVU10的情况下生长后，嗜热栖热菌微菌落的形态有所不同。使用共聚焦激光显微镜可以看到菌落密度较低，并且可以看到嗜热栖热菌的链。在存在对照抗体的情况下生长后，菌落密度高，未观察到长链。得出的结论是，pAbs在肉汤中和在羟基磷灰石圆盘上影响嗜热栖热菌的链长度以及在羟基磷灰石圆盘上的菌落形态，而OMVU10影响嗜热栖热菌在羟基磷灰石圆盘上的菌落形态。
