van Raamsdonk M, de Soet J J, Bosch-Tijhof C J, de Graaff J
Department of Oral Microbiology, Academic Centre for Dentistry Amsterdam, The Netherlands.
Oral Microbiol Immunol. 1996 Aug;11(4):254-8. doi: 10.1111/j.1399-302x.1996.tb00178.x.
The effect of a polyclonal antiserum and OMVU10, a monoclonal antibody reactive with Antigen B of Streptococcus sobrinus, on the interaction of polymorphonuclear leukocytes with S. sobrinus was studied, using chemiluminescence and bacterial killing assays. Increased stimulation of neutrophils as measured in the chemiluminescence assays was established when S. sobrinus was preincubated with polyclonal antiserum or when polyclonal antiserum was added to the reaction mixture. Higher counts were measured in comparison to preimmune serum. After 90 min, 52% of S. sobrinus preincubated with polyclonal antiserum was killed. Killing was also increased when polyclonal antiserum was added to the reaction mixture in comparison to the controls. No killing was found when bacteria were preincubated with OMVU10 or when OMVU10 was added to the reaction mixture in comparison to Clone 24, a control antibody.
使用化学发光和细菌杀伤试验，研究了多克隆抗血清以及与远缘链球菌抗原B反应的单克隆抗体OMVU10对多形核白细胞与远缘链球菌相互作用的影响。当远缘链球菌与多克隆抗血清预孵育或向反应混合物中加入多克隆抗血清时，化学发光试验中测得的中性粒细胞刺激增加。与免疫前血清相比，计数更高。90分钟后，与多克隆抗血清预孵育的远缘链球菌中有52%被杀死。与对照相比，当向反应混合物中加入多克隆抗血清时，杀伤也增加。与对照抗体克隆24相比，当细菌与OMVU10预孵育或向反应混合物中加入OMVU10时，未发现杀伤作用。
