来自单体尾索动物柄海鞘的一种体液调理素。

A humoral opsonin from the solitary urochordate Styela clava.

作者信息

Kelly K L, Cooper E L, Raftos D A

机构信息

Department of Anatomy and Cell Biology, School of Medicine, University of California, Los Angeles 90024.

出版信息

Dev Comp Immunol. 1993 Jan-Feb;17(1):29-39. doi: 10.1016/0145-305x(93)90013-g.

DOI:10.1016/0145-305x(93)90013-g

PMID:8449249

Abstract

Opsonins play a key role in invertebrate humoral immune systems. An opsonin for yeast was identified in the plasma of the tunicate, Styela clava. In vitro cultures of hemocytes with homologous plasma-incubated yeast exhibited significantly higher levels of phagocytosis than controls. Studies indicated that the opsonic activity of Styela clava plasma increased the overall capacity for phagocytosis. Opsonization was inhibited by the carbohydrates mannan, N-acetyl-D-galactosamine, and galactose, and by the divalent cation chelator, EDTA. These data suggest that the Styela clava opsonin may share some functional similarities with a C-type lectin. Incubation of yeast with Styela clava and Styela plicata plasma prior to phagocytosis by hemocytes from both species indicated the Styela clava opsonin is species specific.

摘要

调理素在无脊椎动物体液免疫系统中发挥关键作用。在被囊动物柄海鞘的血浆中鉴定出一种针对酵母的调理素。用同源血浆孵育酵母后的血细胞体外培养物，其吞噬作用水平显著高于对照组。研究表明，柄海鞘血浆的调理活性提高了整体吞噬能力。甘露聚糖、N-乙酰-D-半乳糖胺和半乳糖等碳水化合物以及二价阳离子螯合剂乙二胺四乙酸（EDTA）可抑制调理作用。这些数据表明，柄海鞘调理素可能与C型凝集素具有一些功能相似性。在用来自这两个物种的血细胞进行吞噬作用之前，将酵母与柄海鞘和皱瘤海鞘的血浆一起孵育，结果表明柄海鞘调理素具有物种特异性。