Gallego M, Olah I, Del Cacho E, Glick B
Poultry Science Department, Clemson University, SC 29634-0379.
Dev Comp Immunol. 1993 Jan-Feb;17(1):77-83. doi: 10.1016/0145-305x(93)90017-k.
The chicken spleen was studied immunohistochemically with anti-S-100 protein polyclonal antibody. S-100-positive cells accumulated around the penicilliform capillaries during the first 3 weeks of life. After 2 weeks posthatch the S-100-positive cells appeared in the red pulp, periarterial lymphatic sheath, and subsequently in the germinal center. Their ontogenetic development and intrasplenic distribution strongly suggested that the S-100-positive cells were identical with ellipsoid-associated cells. The S-100-negative cells of the periellipsoidal white pulp gradually transformed to S-100-positive, functionally active cells on the surface of the ellipsoid. The immunohistological findings support the hypothesis that the interdigitating dendritic cells and follicular dendritic cells were not of monocytic origin but belong to a splenic resident, endocytic cell line located on the surface of the ellipsoid.
用抗S-100蛋白多克隆抗体对鸡脾脏进行免疫组织化学研究。在出生后的前3周，S-100阳性细胞聚集在笔形毛细血管周围。孵化后2周，S-100阳性细胞出现在红髓、动脉周围淋巴鞘，随后出现在生发中心。它们的个体发育和脾内分布强烈表明，S-100阳性细胞与椭球体相关细胞相同。椭球体周围白髓的S-100阴性细胞逐渐转化为椭球体表面的S-100阳性、功能活跃的细胞。免疫组织学结果支持这样的假说，即交错突细胞和滤泡树突状细胞不是单核细胞起源，而是属于位于椭球体表面的脾脏固有内吞细胞系。
