Cote R J, Morrissey D M, Houghton A N, Thomson T M, Daly M E, Oettgen H F, Old L J
Proc Natl Acad Sci U S A. 1986 May;83(9):2959-63. doi: 10.1073/pnas.83.9.2959.
Over 4350 human Ig-secreting hybrids have been generated through the fusion of human lymphocytes with NS-1 (mouse), LICR-2, SKO-007, GM4672, or UC729-6 (human) myeloma and lymphoblastoid cell lines. NS-1 proved to be the most satisfactory fusion partner, and 83% of the stable Ig-secreting clones were derived from NS-1 fusions. Three hundred five hybrids produced human monoclonal antibodies (hmAb) reactive with cell surface or intracellular antigens expressed by cultured human tumor cell lines, and 111 of these have undergone detailed serological specificity analysis. Several general points have emerged from our study of hmAb: A significant proportion of the human B-cell clones produce antibody reactive with cellular antigens. The majority of these antigens have an intracellular location and are broadly distributed. Intracellular and cell surface differentiation antigens and other antigens with restricted distribution have been defined by hmAb, including two cell surface antigens not detected on normal cells. The relationship of these findings to cancer is unclear, as hmAb reactive with antigens showing distinctive distribution have been generated from the lymphocytes of normal individuals as well as tumor-bearing patients.
通过将人淋巴细胞与NS-1（小鼠）、LICR-2、SKO-007、GM4672或UC729-6（人）骨髓瘤细胞系和淋巴母细胞系融合，已产生了超过4350个人Ig分泌杂交细胞。事实证明，NS-1是最理想的融合伙伴，83%的稳定Ig分泌克隆源自NS-1融合。305个杂交细胞产生了与培养的人肿瘤细胞系表达的细胞表面或细胞内抗原反应的人单克隆抗体（hmAb），其中111个已进行了详细的血清学特异性分析。我们对hmAb的研究得出了几个一般要点：相当一部分人B细胞克隆产生与细胞抗原反应的抗体。这些抗原大多数位于细胞内，分布广泛。通过hmAb已确定了细胞内和细胞表面分化抗原以及其他分布受限的抗原，包括在正常细胞上未检测到的两种细胞表面抗原。这些发现与癌症的关系尚不清楚，因为从正常个体以及肿瘤患者的淋巴细胞中都产生了与显示独特分布的抗原反应的hmAb。
