Miyamoto K, Nomura M, Sasakura M, Matsui E, Koshiura R, Murayama T, Furukawa T, Hatano T, Yoshida T, Okuda T
Research Laboratory for Development of Medicine, Hokuriku University, School of Pharmacy, Kanazawa.
Jpn J Cancer Res. 1993 Jan;84(1):99-103. doi: 10.1111/j.1349-7006.1993.tb02790.x.
The antitumor effect of oenothein B, a macrocyclic ellagitannin from Oenothera erythrosepala Bordas, on rodent tumors was studied. Oenothein B exhibited a strong antitumor activity against MM2 ascites tumors upon intraperitoneal administration to the mice before or after the tumor inoculation. The tannin also inhibited the growth of Meth-A solid type tumor in mice. This antitumor effect of the tannin could not be attributed to its direct cytotoxic action on tumor cells, because the cytotoxicity was very weak in the presence of serum protein. When oenothein B was injected into the peritoneal cavity of mice, peritoneal exudate cells, including cytostatic macrophages, were induced. Furthermore, in the in vitro treatment of macrophages from mice and humans, the tannin stimulated release of an interleukin 1 (IL-1)-like activity and IL-1 beta from the cells. These results suggest that oenothein B exerts its antitumor effect through potentiation of the host-immune defense via activation of macrophages.
研究了月见草素B（一种来自红萼月见草的大环鞣花单宁）对啮齿动物肿瘤的抗肿瘤作用。在肿瘤接种前或接种后给小鼠腹腔注射月见草素B，其对MM2腹水瘤表现出强烈的抗肿瘤活性。该单宁还抑制小鼠体内Meth-A实体瘤的生长。单宁的这种抗肿瘤作用不能归因于其对肿瘤细胞的直接细胞毒性作用，因为在血清蛋白存在的情况下细胞毒性非常弱。当将月见草素B注入小鼠腹腔时，会诱导包括抑制性巨噬细胞在内的腹腔渗出细胞。此外，在对小鼠和人类巨噬细胞的体外处理中，该单宁刺激细胞释放白细胞介素1（IL-1）样活性物质和IL-1β。这些结果表明，月见草素B通过激活巨噬细胞增强宿主免疫防御来发挥其抗肿瘤作用。
