Venkatesh T V, Radding C M
Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510.
J Bacteriol. 1993 Mar;175(6):1844-6. doi: 10.1128/jb.175.6.1844-1846.1993.
The red genes of bacteriophage lambda specify two proteins, exonuclease and beta protein, which are essential for general recombination of lambda in recA cells. Earlier studies suggested that these proteins form an equimolar complex (C. M. Radding, J. Rosenweig, F. Richards, and E. Cassuto, J. Biol. Chem. 246:2510-2512, 1971). A more recent study indicated that beta protein forms a strong complex with an unknown polypeptide of 70 kDa (K. Muniyappa and C. M. Radding, J. Biol. Chem. 261:7472-7478, 1986). In the present study, in addition to the complex of beta and the 70-kDa protein, a new association of beta protein with a 65-kDa protein was observed. N-terminal sequencing identified these proteins as host-encoded ribosomal protein S1 and transcription terminator protein NusA.
噬菌体λ的红色基因可编码两种蛋白质，即核酸外切酶和β蛋白，它们对于λ在recA细胞中的一般重组至关重要。早期研究表明，这些蛋白质形成等摩尔复合物（C.M.拉丁、J.罗森weig、F.理查兹和E.卡苏托，《生物化学杂志》246:2510 - 2512，1971）。最近的一项研究表明，β蛋白与一种未知的70 kDa多肽形成强复合物（K.穆尼亚帕和C.M.拉丁，《生物化学杂志》261:7472 - 7478，1986）。在本研究中，除了β蛋白与70 kDa蛋白的复合物外，还观察到β蛋白与一种65 kDa蛋白的新结合。N端测序确定这些蛋白质为宿主编码的核糖体蛋白S1和转录终止蛋白NusA。
