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结核分枝杆菌的MTCY428.08基因编码烟酰胺腺嘌呤二核苷酸合成酶。

The MTCY428.08 gene of Mycobacterium tuberculosis codes for NAD+ synthetase.

作者信息

Cantoni R, Branzoni M, Labò M, Rizzi M, Riccardi G

机构信息

Dipartimento di Genetica e Microbiologia, Università di Pavia, Italy.

出版信息

J Bacteriol. 1998 Jun;180(12):3218-21. doi: 10.1128/JB.180.12.3218-3221.1998.

DOI:10.1128/JB.180.12.3218-3221.1998
PMID:9620974
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC107825/
Abstract

The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD+ synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S-transferase fused and His6-tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCY428.08 gene of M. tuberculosis is the structural gene for NAD+ synthetase.

摘要

结核分枝杆菌的MTCY428.08基因产物与几种NAD⁺合成酶具有序列同源性。MTCY428.08基因被克隆到表达载体pGEX - 4T - 1和pET - 15b中。在大肠杆菌中表达导致谷胱甘肽S - 转移酶融合和His6标记的基因产物过量产生，并对其进行NAD合成酶活性的酶学测定。我们的结果表明，结核分枝杆菌的MTCY428.08基因是NAD⁺合成酶的结构基因。

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本文引用的文献

1
Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation.谷氨酰胺-tRNA合成酶：一种在翻译过程中正确解码谷氨酰胺密码子所需的新型异源三聚体酶。
Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11819-26. doi: 10.1073/pnas.94.22.11819.
2
Crystal structure of NH3-dependent NAD+ synthetase from Bacillus subtilis.来自枯草芽孢杆菌的依赖氨的NAD⁺合成酶的晶体结构。
EMBO J. 1996 Oct 1;15(19):5125-34.
3
An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae.结核分枝杆菌H37Rv的基因组整合图谱以及与麻风分枝杆菌的比较。
Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):3132-7. doi: 10.1073/pnas.93.7.3132.
4
The crystal structure of GMP synthetase reveals a novel catalytic triad and is a structural paradigm for two enzyme families.GMP合成酶的晶体结构揭示了一种新型催化三联体，是两个酶家族的结构范例。
Nat Struct Biol. 1996 Jan;3(1):74-86. doi: 10.1038/nsb0196-74.
5
Functional analysis of the outB gene of Bacillus subtilis.枯草芽孢杆菌outB基因的功能分析
J Gen Microbiol. 1993 Jan;139(1):31-7. doi: 10.1099/00221287-139-1-31.
6
The amidotransferases.酰胺转移酶
Adv Enzymol Relat Areas Mol Biol. 1993;66:203-309. doi: 10.1002/9780470123126.ch5.
7
The Escherichia coli efg gene and the Rhodobacter capsulatus adgA gene code for NH3-dependent NAD synthetase.大肠杆菌的efg基因和荚膜红细菌的adgA基因编码氨依赖性NAD合成酶。
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8
Issues in searching molecular sequence databases.搜索分子序列数据库中的问题。
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9
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J Biol Chem. 1995 Mar 17;270(11):6181-5. doi: 10.1074/jbc.270.11.6181.
10
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Microbiol Rev. 1980 Mar;44(1):83-105. doi: 10.1128/mr.44.1.83-105.1980.