结核分枝杆菌的MTCY428.08基因编码烟酰胺腺嘌呤二核苷酸合成酶。
The MTCY428.08 gene of Mycobacterium tuberculosis codes for NAD+ synthetase.
作者信息
Cantoni R, Branzoni M, Labò M, Rizzi M, Riccardi G
机构信息
Dipartimento di Genetica e Microbiologia, Università di Pavia, Italy.
出版信息
J Bacteriol. 1998 Jun;180(12):3218-21. doi: 10.1128/JB.180.12.3218-3221.1998.
Abstract
The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD+ synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S-transferase fused and His6-tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCY428.08 gene of M. tuberculosis is the structural gene for NAD+ synthetase.
摘要
结核分枝杆菌的MTCY428.08基因产物与几种NAD⁺合成酶具有序列同源性。MTCY428.08基因被克隆到表达载体pGEX - 4T - 1和pET - 15b中。在大肠杆菌中表达导致谷胱甘肽S - 转移酶融合和His6标记的基因产物过量产生，并对其进行NAD合成酶活性的酶学测定。我们的结果表明，结核分枝杆菌的MTCY428.08基因是NAD⁺合成酶的结构基因。
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