Adnan S, Li N, Miura H, Hashimoto Y, Yamamoto H, Ezaki T
Department of Microbiology, Gifu University, School of Medicine, Japan.
FEMS Microbiol Lett. 1993 Jan 15;106(2):139-42. doi: 10.1111/j.1574-6968.1993.tb05949.x.
Single-stranded chromosomal DNA was covalently bound to a microdilution plate and used for quantitative DNA-DNA hybridization. After 30 min, hybridized DNA was quantitatively detected by alkaline phosphatase and a chemiluminescent substrate. This method was successfully used for the rapid identification of viridans streptococci.
单链染色体DNA共价结合到微量稀释板上，并用于定量DNA-DNA杂交。30分钟后，通过碱性磷酸酶和化学发光底物对杂交的DNA进行定量检测。该方法成功用于草绿色链球菌的快速鉴定。
