Lindsay R M, Rourke J T, Reid B D, Linton A L, Gilchrist T, Courtney J, Edwards R O
J Lab Clin Med. 1977 Apr;89(4):724-34.
The role of heparin on platelet--foreign surface interactions was examined by platelet retention studies on acrylonitrile--dimethylaminoethyl methacrylate (AN-DMAEMA) dialysis membranes both with and without the bonding of heparin onto their surfaces. Heparin bonding significantly reduced platelet retention. Heparin in solution (4 units/ml.) increased platelet retention when the surface of the membranes was modified by ethylene oxide but had no significant effect on the platelet-retaining properties of unmodified membranes. Studies using heparin 99mTc demonstrated that unmodified membranes took up heparin from solution whereas ethylene oxide-modified membranes had little such affinity. The heparin bonding process greatly increased the heparin uptake achieved by simple soaking in heparin solution, and the leaching rate was less than 1% at 70 hours. The results indicate that heparin has two antagonistic effects in this platelet-foreign surface interaction: it acts directly on platelets to increase adhesiveness while acting on the foreign surface to reduce platelet retention.
通过在丙烯腈 - 甲基丙烯酸二甲氨基乙酯（AN - DMAEMA）透析膜上进行血小板滞留研究，考察了肝素在血小板与异物表面相互作用中的作用，这些透析膜表面有无肝素结合。肝素结合显著降低了血小板滞留。当膜表面用环氧乙烷改性时，溶液中的肝素（4单位/毫升）增加了血小板滞留，但对未改性膜的血小板保留特性没有显著影响。使用肝素99mTc的研究表明，未改性膜从溶液中摄取肝素，而环氧乙烷改性膜几乎没有这种亲和力。肝素结合过程大大增加了通过简单浸泡在肝素溶液中实现的肝素摄取，并且在70小时时浸出率小于1%。结果表明，肝素在这种血小板与异物表面相互作用中具有两种拮抗作用：它直接作用于血小板以增加粘附性，同时作用于异物表面以减少血小板滞留。
