Rustemeier K, Demetriou D, Schepers G, Voncken P
INBIFO Institut für Biologische Forschung, Köln, Germany.
J Chromatogr. 1993 Mar 5;613(1):95-103. doi: 10.1016/0378-4347(93)80201-e.
The 1,3-diethyl-2-thiobarbituric acid (DETBA) assay for nicotine metabolites has been improved so that it can be used to determine the concentrations of nicotine and up to 12 metabolites in the urine of humans and laboratory animals, including phase 2 metabolites. The products of beta-glucuronidase cleavage found in human urine were mainly trans-3'-hydroxycotinine, cotinine, and a small amount of nicotine. Following isolation, spectroscopic analyses showed the structure of the nicotine DETBA derivative to be the one-to-one ring-opening product of DEBTA and the cyanopyridinium salt of nicotine.
用于检测尼古丁代谢物的1,3 - 二乙基 - 2 - 硫代巴比妥酸（DETBA）测定法已得到改进，使其可用于测定人类和实验动物尿液中尼古丁及多达12种代谢物的浓度，包括第二阶段代谢物。在人类尿液中发现的β - 葡萄糖醛酸酶裂解产物主要是反式 - 3'-羟基可替宁、可替宁和少量尼古丁。分离后，光谱分析表明尼古丁DETBA衍生物的结构是DEBTA与尼古丁氰吡啶鎓盐的一对一开环产物。
