Hirsh A V, Mills C, Tan S L, Bekir J, Rainsbury P
Hallam Medical Centre, London, UK.
Hum Reprod. 1993 Jan;8(1):89-90. doi: 10.1093/oxfordjournals.humrep.a137882.
Aspiration of the vas deferens under local anaesthesia was carried out in a man with ejaculatory failure due to tetraplegia after rectal electrostimulation had failed to provide an ejaculate satisfactory for use in in-vitro fertilization. After passage through a discontinuous Percoll gradient and exposure to 3 mmol pentoxifylline and 3 mmol 2-deoxyadenosine, the final preparation consisted of 50,000 spermatozoa with 4% progressive motility. This was sufficient for the insemination of seven of the 23 oocytes that were collected from the patient's wife by transvaginal ultrasound-guided follicular aspiration. Three high-grade embryos were produced which were transferred transcervically into the uterus 2 days later. A singleton pregnancy resulted which is currently ongoing at 30 weeks gestation. Aspiration of the vas deferens is a relatively simple technique which provides an acceptable alternative to rectal electrostimulation, artificial sperm reservoirs and donor insemination in patients with ejaculatory failure.
对于一名因四肢瘫痪而射精失败的男性，在直肠电刺激未能获取用于体外受精的满意精液后，在局部麻醉下进行了输精管抽吸。经过不连续的 Percoll 梯度处理并暴露于 3 mmol 己酮可可碱和 3 mmol 2-脱氧腺苷后，最终制备的精液含有 50,000 个精子，其中 4% 具有进行性运动能力。这足以对通过经阴道超声引导下卵泡抽吸术从患者妻子体内采集的 23 个卵母细胞中的 7 个进行授精。产生了 3 个优质胚胎，2 天后经宫颈将其移植到子宫内。结果是单胎妊娠，目前妊娠已达 30 周。输精管抽吸是一种相对简单的技术，对于射精失败的患者而言，它为直肠电刺激、人工储精库和供体授精提供了一种可接受的替代方法。
