Rohlman V C, Kuhls T L, Mosier D A, Crawford D L, Greenfield R A
Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City.
J Parasitol. 1993 Apr;79(2):295-7.
The role of natural killer (NK) cell activity in adult mice with severe combined immunodeficiency (scid mice) infected with Cryptosporidium parvum oocytes was evaluated. Adult BALB/c and scid mice were inoculated intragastrically with 10(6) C. parvum oocysts after the administration of anti-asialo-GM1 or control normal rabbit serum. Groups of animals were evaluated for splenic NK cell activity and examined histopathologically at 2, 4, and 6 wk postinfection. Virtual elimination of splenic NK cell activity by anti-asialo-GM1 treatment was demonstrated. Nonetheless, no differences in the occurrence of illness, death, or histopathologic evidence of infection were observed between anti-asialo-GM1-treated and control-treated BALB/c or scid mice. We conclude that NK cell activity, at least as measured in the spleen, does not play a significant role in murine host defense of cryptosporidial infection, even in the absence of functional B and T cells.
评估了自然杀伤（NK）细胞活性在感染微小隐孢子虫卵囊的重度联合免疫缺陷成年小鼠（scid小鼠）中的作用。成年BALB/c小鼠和scid小鼠在给予抗去唾液酸GM1或对照正常兔血清后，经胃内接种10(6)个微小隐孢子虫卵囊。在感染后2、4和6周，对动物组进行脾NK细胞活性评估，并进行组织病理学检查。结果表明，抗去唾液酸GM1处理可使脾NK细胞活性几乎完全消除。然而，在抗去唾液酸GM1处理的和对照处理的BALB/c或scid小鼠之间，未观察到疾病发生、死亡或感染的组织病理学证据方面的差异。我们得出结论，即使在缺乏功能性B细胞和T细胞的情况下，NK细胞活性（至少在脾脏中测量）在小鼠宿主对隐孢子虫感染的防御中也不发挥重要作用。
