Zöller M, Matzku S, Schulz U
J Natl Cancer Inst. 1977 Apr;58(4):897-904. doi: 10.1093/jnci/58.4.897.
Peripheral blood leukocytes from patients with gastric cancer and various other malignant and nonmalignant diseases and peripheral blood leukocytes from apparently healthy volunteers were tested in the leukocyte migration inhibition test with the use of 4-5 different 3 M KCl extracts of gastric cancer tissue. An operational criterion for defining sensitization of patients' leukocytes was developed; i.e., evidence in an individual sample of leukocytes of either decreased or increased migration areas (migration index less than or equal to 0.79 and larger than or equal to 1.20, respectively) with 3 or more antigen extracts. With this as an indicator of sensitization, it was found that 91% of patients with gastric cancer (39/43), comparto 5% (5/94) and 3% (1/32) of patients with nonmalignant, nongastric diseases and normal controls, respectively, were reactive. Patients with various nongastric cancers were sensitized in 36% (49/135) of cases. Gastritis and gastric, as well as duodenal, ulcer did not influence the reactivity of patients' leukocytes, but considerable sensitization was found in patients with atrophic gastritis or intestinalization. When classified in the usual manner, i.e., by considering the reaction with individual tumor extracts, the specificity and the sensitivity of the test was markedly diminished: More false negative determinations were found in the group of gastric cancer patients, and the percentage of false positives in the group of nonmalignant diseases increased. The results gave evidence of tumor-associated antigens in gastric cancer patients, against which the host elicited a cellular immune response. The high incidence of positive reactivity of leukocytes from patients with gastric cancer, together with the considerable cross-reactivity of leukocytes from patients with nongastric cancer, pointed to the expression of antigens with organ-related and widespread specificities.
利用胃癌组织的4 - 5种不同的3M氯化钾提取物，对胃癌患者、其他各种恶性和非恶性疾病患者的外周血白细胞以及明显健康志愿者的外周血白细胞进行白细胞迁移抑制试验。制定了一个定义患者白细胞致敏的操作标准；即，在单个白细胞样本中，若有3种或更多抗原提取物的迁移面积减少或增加（迁移指数分别小于或等于0.79和大于或等于1.20），则为致敏证据。以此作为致敏指标，发现91%的胃癌患者（39/43）有反应，而非恶性非胃癌疾病患者和正常对照分别为5%（5/94）和3%（1/32）。各种非胃癌患者中36%（49/135）致敏。胃炎、胃溃疡以及十二指肠溃疡并不影响患者白细胞的反应性，但在萎缩性胃炎或肠化生患者中发现有相当程度的致敏。按常规方式分类，即考虑与单个肿瘤提取物的反应时，该试验的特异性和敏感性明显降低：胃癌患者组中出现更多假阴性结果，非恶性疾病组中的假阳性百分比增加。结果表明胃癌患者体内存在肿瘤相关抗原，宿主针对这些抗原引发了细胞免疫反应。胃癌患者白细胞阳性反应的高发生率，以及非胃癌患者白细胞的相当程度的交叉反应，表明存在具有器官相关和广泛特异性的抗原表达。
