Lanka S T, Klein M, Ramsperger U, Müller D G, Knippers R
Division of Biology, Universität Konstanz, Germany.
Virology. 1993 Apr;193(2):802-11. doi: 10.1006/viro.1993.1189.
We describe a procedure for the isolation of virus particles from the marine brown alga Ectocarpus siliculosus. Virus particles are composed of at least 13 different polypeptides, including two glycoproteins, and double-stranded DNA. A typical virus DNA preparation contains three fractions, namely linear DNA and circular DNA, each composed of about 320 kilobase pairs, as well as DNA fragments, 10 to 60 kilobase pairs in size. The large linear and the circular DNA contain single-stranded regions (average length: 2.9 kilobase). We propose that the native Ectocarpus virus genome is a circular DNA molecule whose double strand is interrupted by single-stranded regions. During the preparation procedure, the DNA circles tend to break at the single-stranded sites producing large linear as well as fragmented DNA.
我们描述了一种从海洋褐藻硅鞭藻中分离病毒颗粒的方法。病毒颗粒由至少13种不同的多肽组成，包括两种糖蛋白和双链DNA。典型的病毒DNA制剂包含三个部分，即线性DNA和环状DNA，每一种都由约320千碱基对组成，以及大小为10至60千碱基对的DNA片段。大的线性DNA和环状DNA含有单链区域（平均长度：2.9千碱基）。我们提出，天然的硅鞭藻病毒基因组是一个环状DNA分子，其双链被单链区域打断。在制备过程中，DNA环倾向于在单链位点断裂，产生大的线性DNA以及片段化DNA。
