de Senarclens C F, Pricam C E, Banichahi F D, Vallotton M B
Kidney Int. 1977 Mar;11(3):161-9. doi: 10.1038/ki.1977.24.
When a marked stimulation of the reninangiotensin system induced in the rat by bilateral adrenalectomy and salt-depletion is abruptly blocked by a 48-hr substitution treatment which desoxycorticosterone acetate (DOCA) and saltload, one observes: 1) an absolute increase of a form of renin revealed only after prior acidification (pH, 2.5) of kidney extract before usual incubation at the optimum pH of 6.5 and 2) the simultaneous appearance of many granules containing crystalline cores in the epithelioid cells of the juxtaglomerular apparatus. These observations are compatible with the existence of two forms of renal renin and show that after renin-depletion of the kidney, newly synthesized renin is mainly in the acid-activated form. Storage of this renin the epitheloid cells may possibly take the form of secretory granules with crystalline cores. It is speculated that the acid-activated from of renin may be either a renin proenzyme or a protein-bound form.
当通过双侧肾上腺切除术和限盐在大鼠中诱导出显著的肾素-血管紧张素系统刺激后，用醋酸去氧皮质酮（DOCA）和盐负荷进行48小时的替代治疗突然阻断该刺激时，人们会观察到：1）一种仅在肾脏提取物在6.5的最佳pH值下常规孵育前先酸化（pH 2.5）后才显示出的肾素形式出现绝对增加，以及2）在近球小体的上皮样细胞中同时出现许多含有结晶核心的颗粒。这些观察结果与存在两种形式的肾肾素相符合，并表明在肾脏肾素耗竭后，新合成的肾素主要呈酸激活形式。这种肾素在上皮样细胞中的储存可能采取具有结晶核心的分泌颗粒形式。据推测，肾素的酸激活形式可能是肾素原酶或蛋白质结合形式。
