Röllinghoff W, Brandes J W, Ehms H, Miller B, Schmülling R M, Tischendorf F W, Malchow H
Klin Wochenschr. 1977 Mar 1;55(5):225-30. doi: 10.1007/BF01487715.
Lysozyme concentrations in serum and urine were determined in 101 patients with Crohn's disease and 26 patients with ulcerative colitis. Lysozyme was assayed according to the lysoplate method of Osserman against a standard of humam lysozyme. The mean serum lysozyme concentrations (+/- S.E.M.) for each group were as follows: controls 8.4 +/- 1.8 (n equals 38), Crohn's disease 8.2+/-2.6 (n equals 101), ulcerative colitis 8.7+/-3.0 (n equals 26). No significant differences were found in serum lysozyme levels of the various groups of patients (2p is greater 0.05). There existed no correlation (r equals 0.12, n equals 129, p is greater than 0.05) with the activity of the disease. Serum lysozyme levels were significantly higher in patients affected by Crohn's disease of the small and the large bowel than in patients with involvement of the small intestine only and operated patients (2p is less than 0.05). The discriminative value of these findings with respect to the clinical course of such patients is limited because no significant differences are found between the levels of patients with Crohn's disease and controls. Neither in case of Crohn's disease nor ulcerative colitis were the mean urine lysozyme concentrations increased. These findings show that the determination of serum and urine lysozyme levels is unsuitable in respect of the differential diagnosis of inflammatory bowel disease as well as of the assessment of activity and extent of the disease.
对101例克罗恩病患者和26例溃疡性结肠炎患者测定了血清和尿液中的溶菌酶浓度。根据奥瑟曼的溶菌平板法，以人溶菌酶标准品对溶菌酶进行测定。每组的平均血清溶菌酶浓度（±标准误）如下：对照组8.4±1.8（n = 38），克罗恩病组8.2±2.6（n = 101），溃疡性结肠炎组8.7±3.0（n = 26）。各患者组的血清溶菌酶水平未发现显著差异（P>0.05）。与疾病活动度不存在相关性（r = 0.12，n = 129，P>0.05）。累及小肠和大肠的克罗恩病患者的血清溶菌酶水平显著高于仅累及小肠的患者和接受手术的患者（P<0.05）。这些发现对于此类患者临床病程的鉴别价值有限，因为克罗恩病患者与对照组的水平未发现显著差异。无论是克罗恩病还是溃疡性结肠炎，尿溶菌酶平均浓度均未升高。这些发现表明，血清和尿液溶菌酶水平的测定对于炎症性肠病的鉴别诊断以及疾病活动度和范围的评估均不合适。
