Pruzanski W, Marcon N, Ottaway C, Prokipchuk E
Am J Dig Dis. 1977 Nov;22(11):995-8. doi: 10.1007/BF01076199.
Estimation of lysozyme (LZM) activity in the serum was suggested as a valuable test to distinguish between Crohn's disease and ulcerative colitis. Subsequently several reports either supported or denied the original observation. Selection of patients and methodological differences were suggested as an explanation for the controversy. We estimated serum LZM in a large group of patients using the lysoplate method and human LZM as a standard. The conditions of the assay were strictly standardized. In 90 patients with Crohn's disease the LZM level was 8.3 +/- 2.1 (SD) microgram/ml, in 57 patients with ulcerative colitis was 7.4 +/- 2.0 (SD) microgram/ml, and in 40 healthy individuals it was 7.0 +/- 1.2 (SD) microgram/ml. Although the difference between the mean LZM levels in Crohn's disease and in ulcerative colitis was statistically significant, there was a definite overlapping of values between these two diseases. No significant correlation of LZM level to the duration or extent of the disease, activity, or treatment was found in Crohn's disease. In ulcerative colitis the LZM level was often a little higher in severe disease, especially when the whole colon was involved.
血清溶菌酶（LZM）活性测定被认为是区分克罗恩病和溃疡性结肠炎的一项有价值的检测方法。随后，有几份报告支持或否定了最初的观察结果。患者的选择和方法学差异被认为是造成争议的原因。我们使用溶菌平板法并以人LZM作为标准，对一大组患者的血清LZM进行了测定。检测条件严格标准化。90例克罗恩病患者的LZM水平为8.3±2.1（标准差）微克/毫升，57例溃疡性结肠炎患者为7.4±2.0（标准差）微克/毫升，40名健康个体为7.0±1.2（标准差）微克/毫升。虽然克罗恩病和溃疡性结肠炎的平均LZM水平差异具有统计学意义，但这两种疾病的数值存在一定重叠。在克罗恩病中，未发现LZM水平与疾病持续时间、范围、活动度或治疗之间存在显著相关性。在溃疡性结肠炎中，严重疾病时LZM水平通常略高，尤其是全结肠受累时。
