Guse A H, Greiner E, Emmrich F, Brand K
Max-Planck Society, Institute for Clinical Immunology, Schwabachanlage, Erlangen, Germany.
J Biol Chem. 1993 Apr 5;268(10):7129-33.
Changes in the cellular mass and the cellular concentration of the highly phosphorylated inositol derivatives inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) and inositol hexakisphosphate (InsP6) were observed during a complete cell cycle of proliferating rat thymocytes. Inositol polyphosphates were determined by a recently developed anion-exchange HPLC/post-column complexometric dye system (Mayr, G. W. (1988) Biochem. J. 254, 585-591), and data were expressed as mass amounts per cell number or as absolute intracellular concentrations based on cell volume determinations by the cell analyzer system CASY 1. After a very early transient rise in both Ins(1,3,4,5,6)P5 and InsP6 at the beginning of the cell cycle, a decrease of the intracellular concentration of both compounds occurred which then remained on a low level between 24 and 48 h. Between 48 and 72 h the cells divided resulting in a decrease of the cell volume by a factor of approximately 2. During this phase a pronounced increase of Ins(1,3,4,5,6)P5 and InsP6 was observed. These increases were already seen when the data were expressed as mass per cell number, but were even enhanced when expressed as absolute intracellular concentrations. In conclusion, we present as a novel finding long term changes in the intracellular concentrations of Ins(1,3,4,5,6)P5 and InsP6 in proliferating thymocytes. This may indicate a role for these compounds during cell cycle progression.
在增殖大鼠胸腺细胞的完整细胞周期中，观察到细胞质量以及高度磷酸化的肌醇衍生物1,3,4,5,6 - 五磷酸肌醇（Ins(1,3,4,5,6)P5）和六磷酸肌醇（InsP6）的细胞浓度的变化。肌醇多磷酸通过最近开发的阴离子交换高效液相色谱/柱后络合比色系统进行测定（迈尔，G. W.（1988年）《生物化学杂志》254卷，585 - 591页），数据表示为每细胞数量的质量量，或基于细胞分析仪系统CASY 1测定的细胞体积表示为绝对细胞内浓度。在细胞周期开始时，Ins(1,3,4,5,6)P5和InsP6都有一个非常早期的短暂升高，随后这两种化合物的细胞内浓度下降，然后在24至48小时之间保持在低水平。在48至72小时之间，细胞分裂，导致细胞体积减小约2倍。在此阶段，观察到Ins(1,3,4,5,6)P5和InsP6有明显增加。当数据表示为每细胞数量的质量时就已经观察到这些增加，但表示为绝对细胞内浓度时增加更为明显。总之，我们提出了一个新发现，即增殖胸腺细胞中Ins(1,3,4,5,6)P5和InsP6的细胞内浓度存在长期变化。这可能表明这些化合物在细胞周期进程中发挥作用。
