Miwa H, Wada R, Abe H, Ohkura R, Yang S W, Watanabe H, Ogihara T, Hamada T, Sato N
Department of Gastroenterology, Juntendo University, School of Medicine, Tokyo, Japan.
J Gastroenterol Hepatol. 1993 Mar-Apr;8(2):133-7. doi: 10.1111/j.1440-1746.1993.tb01503.x.
Differentiation between gastric adenomas and type IIa early gastric cancer by macroscopic and histologic examinations is often difficult. This study has shown that there are differences in the distribution of S-phase cells in these two types of tumour, and these differences can be exploited to differentiate between them. The distribution of S-phase cells was assessed by Bromodeoxyuridine (BrdU) immunohistochemistry using an in vitro BrdU-labelling method. Biopsy specimens were obtained from 27 lesions (12 adenomas and 15 type IIa early gastric cancers), and the distributions of BrdU-labelled cells in the tumour thickness as well as in the superficial layer of the tumour were determined. Significant difference was seen in the labelling of the two tumour types with regard to both these parameters. Using the criteria of BrdU immunohistochemistry, the accuracy of differentiation between gastric adenomas and cancers was 88.9%. Therefore, it is concluded in this study that in vitro BrdU labelling is a useful method of diagnosing gastric adenomas and cancers.
通过宏观和组织学检查来鉴别胃腺瘤和Ⅱa型早期胃癌往往很困难。本研究表明，这两种肿瘤的S期细胞分布存在差异，这些差异可用于对它们进行鉴别。采用体外溴脱氧尿苷（BrdU）标记法，通过BrdU免疫组织化学评估S期细胞的分布。从27个病变（12个腺瘤和15个Ⅱa型早期胃癌）获取活检标本，测定肿瘤厚度以及肿瘤表层中BrdU标记细胞的分布。在这两个参数方面，两种肿瘤类型的标记存在显著差异。根据BrdU免疫组织化学标准，胃腺瘤与癌的鉴别准确率为88.9%。因此，本研究得出结论，体外BrdU标记是诊断胃腺瘤和癌的一种有用方法。
