Takyu S
Department of Urology, Akita University School of Medicine.
Nihon Hinyokika Gakkai Zasshi. 1993 Feb;84(2):330-8. doi: 10.5980/jpnjurol1989.84.330.
Effects of testosterone on the autonomic receptor-mediated function of the male rabbit urinary bladder and proximal urethra were examined. The alteration of alpha 1-, alpha 2-, beta-adrenergic receptor and muscarinic cholinergic receptor densities in the urinary bladder and proximal urethra were determined by radioligand binding techniques after castration. The density of alpha 1-adrenergic receptor in the proximal urethra decreased gradually after castration. This change was significant 8 weeks after castration. The alpha 2-adrenergic receptor density in the proximal urethra slightly increased after castration, but it was not statistically significant. The beta-adrenergic receptor density in the bladder dome did not change after castration. The density of muscarinic cholinergic receptor in the bladder dome significantly decreased in animals 2 weeks after castration. Additionally, the density of autonomic receptors and the response of muscle strips to autonomic drugs were compared in three age-matched experimental groups: control group, castrated group and castrated and testosterone supplemented group. When the autonomic receptor densities in the castrated group were compared to those of the control, the changes similar to the result in the previous experiment were noted. Testosterone supplementation restored the densities of alpha 1-adrenergic and muscarinic cholinergic receptors in castrated group to control levels. The responses of muscle strips to autonomic drugs closely paralleled the receptor densities. Castration decreased the alpha 1-adrenergic and the muscarinic cholinergic receptor function, however, testosterone restored those function. These findings indicate that testosterone affects the autonomic receptor-mediated function in the smooth muscle of lower urinary tract.
研究了睾酮对雄性兔膀胱和近端尿道自主神经受体介导功能的影响。阉割后，采用放射性配体结合技术测定膀胱和近端尿道中α1-、α2-、β-肾上腺素能受体及毒蕈碱型胆碱能受体密度的变化。阉割后近端尿道α1-肾上腺素能受体密度逐渐降低，阉割8周后这种变化显著。阉割后近端尿道α2-肾上腺素能受体密度略有增加，但无统计学意义。阉割后膀胱顶部β-肾上腺素能受体密度无变化。阉割2周后动物膀胱顶部毒蕈碱型胆碱能受体密度显著降低。此外，在三个年龄匹配的实验组中比较了自主神经受体密度和肌肉条对自主神经药物的反应：对照组、阉割组和阉割并补充睾酮组。当将阉割组的自主神经受体密度与对照组进行比较时，发现了与先前实验结果相似的变化。补充睾酮可使阉割组的α1-肾上腺素能受体和毒蕈碱型胆碱能受体密度恢复到对照水平。肌肉条对自主神经药物的反应与受体密度密切相关。阉割降低了α1-肾上腺素能和毒蕈碱型胆碱能受体功能，然而，睾酮恢复了这些功能。这些发现表明，睾酮影响下尿路平滑肌中自主神经受体介导的功能。
