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含硫醚连接二核苷的DNA的固相合成及杂交性质

Solid-phase synthesis and hybridization properties of DNA containing sulfide-linked dinucleosides.

作者信息

Kawai S H, Wang D, Giannaris P A, Damha M J, Just G

机构信息

Department of Chemistry, McGill University, Montreal, Quebec, Canada.

出版信息

Nucleic Acids Res. 1993 Mar 25;21(6):1473-9. doi: 10.1093/nar/21.6.1473.

DOI:10.1093/nar/21.6.1473
PMID:8464740
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC309335/
Abstract

Oligodeoxyribonucleotides incorporating non-hydrolyzable dialkyl sulfide linked thymidine dimers (TsT) were synthesized chemically by the solid-phase approach. The sulfide dimer TsT was stable to degradation by snake-venom phosphodiesterase, calf spleen phosphodiesterase, Nuclease P1 and Nuclease S1. Thermal denaturation analysis indicated that the incorporation of TsT dimers into DNA weakened, but did not prevent, binding to complementary DNA and RNA over a wide range of salt concentrations (10 mM to 2 M NaCl).

摘要

通过固相方法化学合成了含有不可水解的二烷基硫醚连接的胸腺嘧啶二聚体（TsT）的寡脱氧核糖核苷酸。硫化物二聚体TsT对蛇毒磷酸二酯酶、小牛脾磷酸二酯酶、核酸酶P1和核酸酶S1的降解具有稳定性。热变性分析表明，在广泛的盐浓度范围（10 mM至2 M NaCl）内，将TsT二聚体掺入DNA会削弱但不会阻止与互补DNA和RNA的结合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdd8/309335/48011c6d504e/nar00055-0142-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdd8/309335/599829f06fa7/nar00055-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdd8/309335/48011c6d504e/nar00055-0142-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdd8/309335/599829f06fa7/nar00055-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdd8/309335/48011c6d504e/nar00055-0142-b.jpg

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本文引用的文献

1
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Biochemistry. 1982 Oct 26;21(22):5468-74. doi: 10.1021/bi00265a014.
2
Synthetic analogues of polynucleotides. IX. Synthesis of 3'-O-carboxymethyl-2'-deoxyribonucleosides and their use in the synthesis of an analogue of 2'-deoxyadenylyl-(3' leads to 5'-)-thymidine 3'-phosphate.多核苷酸的合成类似物。IX. 3'-O-羧甲基-2'-脱氧核糖核苷的合成及其在2'-脱氧腺苷酰-(3'→5')-胸苷3'-磷酸类似物合成中的应用。
J Chem Soc Perkin 1. 1973;3:290-4.
3
Inhibition of rabbit globin mRNA translation by sequence-specific oligodeoxyribonucleotides.
含烷基硫代核苷间连接键和2'-取代3'-脱氧核糖核苷的寡核苷酸类似物RNA结合选择性的结构基础
Nucleic Acids Res. 1995 Oct 11;23(19):3967-73. doi: 10.1093/nar/23.19.3967.
序列特异性寡脱氧核糖核苷酸对兔珠蛋白mRNA翻译的抑制作用。
Biochemistry. 1985 Oct 22;24(22):6132-8. doi: 10.1021/bi00343a015.
4
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J Biol Chem. 1986 Jan 15;261(2):592-8.
5
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7
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8
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9
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Nucleic Acids Res. 1988 Jun 10;16(11):4831-9. doi: 10.1093/nar/16.11.4831.
10
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Nucleic Acids Res. 1988 May 25;16(10):4583-94. doi: 10.1093/nar/16.10.4583.