Froehler B, Ng P, Matteucci M
Department of Molecular Biology, Genentech, Inc., South San Francisco, CA 94080.
Nucleic Acids Res. 1988 Jun 10;16(11):4831-9. doi: 10.1093/nar/16.11.4831.
The deoxyoligonucleotide 5' AATCGGGCATGGATT (15-mer) was synthesized containing 12 phosphoramidate linkages derived from 2 primary and 2 secondary amines. The oligonucleotides were purified by reverse phase HPLC and characterized by PAGE. The thermal stability of the duplexes derived from these compounds, when hybridized to the complementary diester linked oligomer, were determined and compared to the diester and methanephosphonate oligomer. The results indicated that all analogue oligomers form less stable duplexes then the diester oligomer. A large difference was observed between primary and secondary amine derived phosphoramidates.
合成了脱氧寡核苷酸5'AATCGGGCATGGATT（15聚体），其含有12个源自2个伯胺和2个仲胺的氨基磷酸酯键。寡核苷酸通过反相高效液相色谱法纯化，并通过聚丙烯酰胺凝胶电泳进行表征。测定了这些化合物衍生的双链体与互补的二酯连接寡聚物杂交时的热稳定性，并与二酯和甲膦酸酯寡聚物进行比较。结果表明，所有类似物寡聚物形成的双链体稳定性均低于二酯寡聚物。在伯胺和仲胺衍生的氨基磷酸酯之间观察到很大差异。
