Jeffery H, Davis S S, O'Hagan D T
Department of Pharmaceutical Sciences, University of Nottingham, United Kingdom.
Pharm Res. 1993 Mar;10(3):362-8. doi: 10.1023/a:1018980020506.
Poly(lactide-co-glycolide) (PLG) microparticles with entrapped antigens have recently been investigated as controlled-release vaccines. This paper describes the preparation of PLG microparticles with an entrapped model antigen, ovalbumin (OVA), using a (water-in-oil)-in-water emulsion solvent evaporation technique. In a series of experiments, the effects of process parameters on particle size and OVA entrapment were investigated. It was found that smooth, spherical microparticles 1-2 microns in diameter containing up to 10% (w/w) OVA could be produced using a small volume of external aqueous phase containing a high concentration of emulsion stabilizer and a 1:5 antigen:polymer ratio. PAGE analysis, isoelectric focusing, and Western blotting of OVA released from the microparticles in vitro confirmed that the molecular weight and antigenicity of the protein remained largely unaltered by the entrapment procedure.
包裹有抗原的聚（丙交酯-共-乙交酯）（PLG）微粒最近已作为控释疫苗进行了研究。本文描述了使用水包油包水乳液溶剂蒸发技术制备包裹有模型抗原卵清蛋白（OVA）的PLG微粒。在一系列实验中，研究了工艺参数对粒径和OVA包封率的影响。结果发现，使用少量含有高浓度乳液稳定剂的外部水相和1:5的抗原：聚合物比例，可以制备出直径为1-2微米、含有高达10%（w/w）OVA的光滑球形微粒。对微粒体外释放的OVA进行的PAGE分析、等电聚焦和蛋白质印迹证实，蛋白质的分子量和抗原性在包封过程中基本保持不变。
