Tabata Y, Ikada Y
Research Center for Medical Polymers and Biomaterials, Kyoto University, Japan.
Biomaterials. 1988 Jul;9(4):356-62. doi: 10.1016/0142-9612(88)90033-6.
Polystyrene and phenylated polyacrolein microspheres of different diameters, as well as modified cellulose microspheres with different surface charges, were prepared in order to study the size and surface charge effect on their phagocytosis by mouse peritoneal macrophages. It was found that the maximal phagocytosis of polystyrene and phenylated polyacrolein microspheres took place when their size was in the range 1.0-2.0 microns. Microspheres with hydrophobic surfaces were more readily phagocytosed than those with hydrophilic surfaces. There was no significant difference in phagocytosis between cationic and the anionic surfaces when compared at a zeta potential of the same absolute value. The least phagocytosis was observed for cellulose microspheres with non-ionic hydrophilic surfaces. Addition of fetal calf serum to the culture medium resulted in decrease in phagocytosis for all microspheres.
制备了不同直径的聚苯乙烯和苯基化聚丙烯醛微球，以及具有不同表面电荷的改性纤维素微球，以研究尺寸和表面电荷对小鼠腹腔巨噬细胞吞噬它们的影响。结果发现，聚苯乙烯和苯基化聚丙烯醛微球在尺寸为1.0 - 2.0微米范围内时吞噬作用最强。具有疏水表面的微球比具有亲水表面的微球更容易被吞噬。当在相同绝对值的zeta电位下比较时，阳离子表面和阴离子表面的吞噬作用没有显著差异。观察到具有非离子亲水表面的纤维素微球吞噬作用最弱。向培养基中添加胎牛血清导致所有微球的吞噬作用降低。
