Ohashi Y, Nakai Y, Ohno Y, Sugiura Y, Okamoto H, Sakamoto H, Hayashi M
Department of Otolaryngology, Osaka City University Medical School, Japan.
Eur Arch Otorhinolaryngol. 1993;250(1):27-32. doi: 10.1007/BF00176944.
A total of 120 Hartley strain guinea pigs were used to investigate the possible role of influenza A in endotoxin-induced otitis media with effusion. Intratympanic inoculation of 0.2 ml physiologic saline solution containing 10(4) plaque-forming units (PFU)/ml of influenza A suspension or 100 ng/ml lipopolysaccharide failed to induce either middle ear effusions or mucociliary pathologies in the tubotympanum. In contrast, intratympanic inoculation 100 ng/ml endotoxin resulted in prolonged mucociliary dysfunction and middle ear effusions when 0.2 ml 10(4) PFU/ml of influenza A was inoculated in the tympanic cavity. The inference is drawn that an influenza A infection might predispose the middle ear to endotoxin-induced otitis media with effusion.
总共120只Hartley品系豚鼠被用于研究甲型流感在脂多糖诱导的分泌性中耳炎中可能发挥的作用。鼓室内接种含有10⁴ 噬斑形成单位（PFU）/毫升甲型流感病毒悬液的0.2毫升生理盐溶液或100纳克/毫升脂多糖，均未诱发中耳积液或鼓室咽鼓管黏膜纤毛病变。相比之下，当鼓室内接种0.2毫升含10⁴ PFU/毫升甲型流感病毒的溶液时，鼓室内接种100纳克/毫升内毒素会导致黏膜纤毛功能障碍持续时间延长及中耳积液。由此推断，甲型流感感染可能使中耳易患脂多糖诱导的分泌性中耳炎。
