矮牵牛富含甘氨酸蛋白1的发育表达与定位
Developmental expression and localization of petunia glycine-rich protein 1.
作者信息
Condit C M
机构信息
Department of Biochemistry, University of Nevada, Reno 89557-0014.
出版信息
Plant Cell. 1993 Mar;5(3):277-88. doi: 10.1105/tpc.5.3.277.
Abstract
An anti-petunia glycine-rich protein 1 (ptGRP1) antibody was used for biotin-streptavidin-alkaline phosphatase localization of this protein. In petunia stem and leaves grown under different light conditions, these studies revealed a complex pattern of cell localization for this protein. Levels of ptGRP1 were shown to decrease with developmental age of the tissue, appearing to correlate directly with expansive growth and inversely with lignification. Significantly, plants grown under low light (approximately 32 mumol m-2 sec-1 at noon) showed at least an eightfold increased level of ptGRP1 protein throughout ptGRP1's expression period when compared to plants grown under higher light (approximately 80 mumol m-2 sec-1 at noon). Evidence also indicated that for one cell type in which ptGRP1 is localized, this protein is imported rather than synthesized. In addition, confocal microscopy studies suggested that ptGRP1 is deposited at the cell wall/membrane interface rather than within the cell wall.
摘要
一种抗矮牵牛富含甘氨酸蛋白1（ptGRP1）抗体用于该蛋白的生物素-链霉亲和素-碱性磷酸酶定位。在不同光照条件下生长的矮牵牛茎和叶中，这些研究揭示了该蛋白复杂的细胞定位模式。结果显示，ptGRP1水平随组织发育年龄降低，似乎与细胞膨大生长直接相关，与木质化呈负相关。值得注意的是，与在较高光照（中午约80 μmol m-2 s-1）下生长的植物相比，在低光照（中午约32 μmol m-2 s-1）下生长的植物在ptGRP1整个表达期内ptGRP1蛋白水平至少增加了八倍。证据还表明，对于ptGRP1定位的一种细胞类型，该蛋白是被转运进来而非合成的。此外，共聚焦显微镜研究表明，ptGRP1沉积在细胞壁/膜界面而非细胞壁内。
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本文引用的文献
1
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Plant Physiol. 1990 Jun;93(2):596-602. doi: 10.1104/pp.93.2.596.
2
Different Temporal and Spatial Gene Expression Patterns Occur during Anther Development.花药发育过程中会出现不同的时空基因表达模式。
Plant Cell. 1990 Dec;2(12):1201-1224. doi: 10.1105/tpc.2.12.1201.
3
Tissue-Specific Expression of Cell Wall Proteins in Developing Soybean Tissues.细胞壁蛋白在发育中的大豆组织中的组织特异性表达
Plant Cell. 1991 Jan;3(1):23-37. doi: 10.1105/tpc.3.1.23.
4
Ultrastructural Localization of a Bean Glycine-Rich Protein in Unlignified Primary Walls of Protoxylem Cells.一种富含甘氨酸的大豆蛋白在原生木质部细胞未木质化初生壁中的超微结构定位
Plant Cell. 1992 Jul;4(7):773-783. doi: 10.1105/tpc.4.7.773.
5
Some new methods for affixing sections to glass slides. I. Aqueous adhesives.将切片固定于载玻片的一些新方法。I. 水性粘合剂。
Stain Technol. 1987 Jan;62(1):27-33. doi: 10.3109/10520298709107962.
6
Bone marrow grafts and tolerance.骨髓移植与耐受性。
Nature. 1986;323(6084):110-1. doi: 10.1038/323110b0.
7
Expression of a gene encoding a glycine-rich protein in petunia.
Mol Cell Biol. 1987 Dec;7(12):4273-9. doi: 10.1128/mcb.7.12.4273-4279.1987.
8
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Plant Cell. 1990 Aug;2(8):785-93. doi: 10.1105/tpc.2.8.785.
9
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10
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EMBO J. 1992 Jun;11(6):2055-62. doi: 10.1002/j.1460-2075.1992.tb05263.x.
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