Arreaza E E, Gibbons J J, Siskind G W, Weksler M E
Department of Medicine, Cornell University Medical College, New York 10021.
Clin Exp Immunol. 1993 Apr;92(1):169-73. doi: 10.1111/j.1365-2249.1993.tb05965.x.
The production of anti-tetanus toxoid (TT) antibody and anti-anti-TT (auto-anti-Id) antibody has been measured in three young and three old persons. Both antibodies were measured using ELISA. We found that: (i) the anti-TT response of old subjects was lower than that of young subjects, (ii) serum auto-anti-Id antibody concentration was higher in old compared with young humans before boosting with TT, and (iii) anti-TT antibodies from different humans shared Id cross-reactivity when tested with a rabbit anti-Id antibody. Thus, the serum anti-TT antibody response to boosting was inversely correlated with the serum level of auto-anti-Id. This conclusion is consistent with the view that the higher level of auto-anti-antibody in older subjects contributes to their impaired antibody response to TT.
我们检测了三名年轻人和三名老年人体内抗破伤风类毒素（TT）抗体及抗抗TT（自身抗独特型）抗体的产生情况。两种抗体均采用酶联免疫吸附测定（ELISA）法进行检测。我们发现：（i）老年受试者的抗TT反应低于年轻受试者；（ii）在使用TT加强免疫前，老年人体内血清自身抗独特型抗体浓度高于年轻人；（iii）用兔抗独特型抗体检测时，不同人体内的抗TT抗体具有独特型交叉反应性。因此，血清中抗TT抗体对加强免疫的反应与自身抗独特型抗体的血清水平呈负相关。这一结论与以下观点一致，即老年受试者体内较高水平的自身抗抗体导致其对TT的抗体反应受损。
