Geha R S
J Immunol. 1982 Jul;129(1):139-44.
Auto-anti-idiotypic antibodies were searched for in the serum of two individuals who received booster immunization with tetanus toxoid (TT) antigen. IgG from serial bleeds obtained for 4 mo after immunization was studied a) for its capacity to specifically bind 125I IgG F(ab')2 anti-TT prepared from serum obtained 7 and 10 days post-booster immunization, and b) for its capacity (after its absorption with TT) to specifically inhibit the binding of 125I-radiolabeled TT to IgG obtained 7 and 10 days post-immunization and designated "peak IgG." In both individuals studied, auto-anti-Id IgG were detected in both assays as early as 2 wk post-booster immunization and remained detectable throughout the study period. Evidence for modulation of idiotypic expression on IgG F(ab')2 anti-TT after booster immunization with TT was obtained by studying a) the capacity of serial IgG samples to inhibit the binding of 125I-IgG F(ab')2 anti-TT to rabbit anti-Id antibody raised against the IgG F(ab')2 anti-TT, and b) the capacity of the rabbit anti-Id to specifically inhibit the binding of 125I-TT to serial samples of serum IgG. Both assays indicated that some idiotypic determinants present on anti-TT obtained before and shortly after booster immunization were expressed to a much lesser extent 2 wk and beyond after immunization. The decrease in the expression of these determinants coincided with the appearance of auto-anti-Id antibody and remained evident throughout the 4-mo study period. The results obtained indicate that auto-anti-Id antibodies arise in normal human subjects after a secondary challenge with antigen and suggest that these antibodies may play a role in the modulation of idiotypic expression that is observed after booster immunization with antigen.
在两名接受破伤风类毒素（TT）抗原加强免疫的个体血清中寻找自身抗独特型抗体。研究了免疫后4个月内连续采血获得的IgG：a）其特异性结合由加强免疫后7天和10天获得的血清制备的125I IgG F(ab')2抗TT的能力；b）其（用TT吸收后）特异性抑制125I放射性标记的TT与免疫后7天和10天获得的并称为“峰值IgG”的IgG结合的能力。在研究的两名个体中，两种检测方法均早在加强免疫后2周就检测到了自身抗独特型IgG，并且在整个研究期间都能检测到。通过研究以下内容获得了用TT加强免疫后IgG F(ab')2抗TT上独特型表达调节的证据：a）连续IgG样本抑制125I-IgG F(ab')2抗TT与针对IgG F(ab')2抗TT产生的兔抗独特型抗体结合的能力；b）兔抗独特型抗体特异性抑制125I-TT与血清IgG连续样本结合的能力。两种检测均表明，加强免疫前及刚免疫后获得的抗TT上存在的一些独特型决定簇在免疫后2周及以后表达程度明显降低。这些决定簇表达的降低与自身抗独特型抗体的出现同时发生，并且在整个4个月的研究期间都很明显。获得的结果表明，正常人类受试者在抗原二次刺激后会产生自身抗独特型抗体，并提示这些抗体可能在抗原加强免疫后观察到的独特型表达调节中起作用。
