链特异性荧光原位杂交揭示18号染色体α卫星DNA的方向。
Strand-specific FISH reveals orientation of chromosome 18 alphoid DNA.
作者信息
Goodwin E, Meyne J
机构信息
Life Sciences Division, Los Alamos National Laboratory, NM 87545.
出版信息
Cytogenet Cell Genet. 1993;63(2):126-7. doi: 10.1159/000133516.
PMID:8467711
Abstract
A method is described for making in situ hybridization strand specific. Through the use of a synthetic DNA probe of a repetitive sequence in the centromeric region of chromosome 18, it is shown that the repeats exist in a head-to-tail tandem array. The method should be useful for studies of the molecular organization and mapping of chromosomes.
摘要
描述了一种使原位杂交具有链特异性的方法。通过使用位于18号染色体着丝粒区域的重复序列的合成DNA探针，表明这些重复序列以头对尾串联阵列的形式存在。该方法对于染色体的分子组织和图谱研究应该是有用的。
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