Kamio Y, Rahman A, Nariya H, Ozawa T, Izaki K
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
FEBS Lett. 1993 Apr 19;321(1):15-8. doi: 10.1016/0014-5793(93)80611-w.
Staphylococcal bi-component toxins, leukocidin and gamma-hemolysin, consist of two protein components, i.e. F and S for leukocidin and H gamma I and H gamma II for gamma-hemolysin. In this study we purified H gamma I and H gamma II to homogeneity from the culture medium of Staphylococcus aureus RIMD 310925 and compared their properties with those of F and S purified from the same source. The N-terminal 59- and C-terminal 2-residue amino acid sequences, apparent molecular mass, and isoelectric point of purified H gamma I were the same as those of F. In an Ouchterlony double diffusion test a fused line without spur was formed between F and H gamma I using either anti-F or anti-H gamma I antibodies. A synergistic action of F and H gamma II caused hemolysis of human red blood cells, and H gamma I acted synergistically with S to exhibit leukocidin activity. We conclude that the two toxins share one protein component (F = H gamma I) in common and leukocidin- and gamma-hemolysin-specific activities are determined by S and H gamma II, respectively. It is also reported that the N-terminal 58-residue sequence of H gamma II is 72% similar to the corresponding sequence of S.
葡萄球菌双组分毒素白细胞毒素和γ-溶血素由两种蛋白质组分组成，即白细胞毒素的F和S组分以及γ-溶血素的HγI和HγII组分。在本研究中，我们从金黄色葡萄球菌RIMD 310925的培养基中纯化了HγI和HγII至均一，并将它们的性质与从同一来源纯化的F和S的性质进行了比较。纯化的HγI的N端59个氨基酸残基序列、C端2个氨基酸残基序列、表观分子量和等电点与F相同。在免疫双扩散试验中，使用抗F或抗HγI抗体时，F和HγI之间形成了无刺的融合线。F和HγII的协同作用导致人红细胞溶血，HγI与S协同作用表现出白细胞毒素活性。我们得出结论，这两种毒素共有一种蛋白质组分（F = HγI），白细胞毒素和γ-溶血素的特异性活性分别由S和HγII决定。另据报道，HγII的N端58个氨基酸残基序列与S的相应序列有72%的相似性。
