来自有和没有冠状动脉疾病受试者的富含和缺乏唾液酸的低密度脂蛋白中蛋白质和脂质成分的碳水化合物组成。

Carbohydrate composition of protein and lipid components in sialic acid-rich and -poor low density lipoproteins from subjects with and without coronary artery disease.

作者信息

Tertov V V, Orekhov A N, Sobenin I A, Morrisett J D, Gotto A M, Guevara J G

机构信息

Institute of Experimental Cardiology, Russian Cardiology Research Center, Moscow.

出版信息

J Lipid Res. 1993 Mar;34(3):365-75.

PMID:8468522

Abstract

Low density lipoprotein (LDL) from patients with coronary heart disease (CHD) caused 78-286% increase in accumulation of cholesterol in human aortic subendothelial cells compared to 2-17% caused by LDL from normal subjects. Ricin-Sepharose affinity chromatography was used to separate LDL into two subfractions, one sialic acid-rich (SAR) and the other sialic acid-poor (SAP). SAP-LDL from CHD patients caused 156-307% increase in accumulation of cellular cholesterol, whereas SAR-LDL from these patients caused only 14-21% increase. SAP-LDL from normal healthy subjects caused 50-86% increased accumulation, whereas their SAR-LDL induced only 2-12% increase. Carbohydrate analysis of SAP-LDL protein isolated from four CHD patients revealed mean values of 59, 25, 61, and 11 nmoles of N-acetyl glucosamine, galactose, mannose, and sialic acid per mg protein, respectively. Mean values for SAR-LDL protein from these patients were 59, 31, 77, and 24 nmol/mg protein, respectively. Analysis of SAP-LDL protein from four normal healthy subjects indicated respective mean values of 58, 29, 72, and 22 nmol/mg, whereas SAR-LDL protein from normals contained 59, 29, 72, and 29 nmol/mg. The carbohydrate content of LDL lipids represents about 25% of the total carbohydrate present in the lipoprotein. The mean values for SAP-LDL lipids from four CHD patients were about 2, 2, 18, 18, and 2 nmol/mg protein for N-acetyl galactosamine, N-acetyl glucosamine, galactose, glucose, and sialic acid, respectively. The mean values for SAR-LDL lipids from these patients were 3, 4, 34, 41, and 5 nmol/mg, respectively. Analysis of SAP-LDL lipids from four normal healthy subjects indicated respective mean values of 4, 6, 30, 31, and 3 nmol/mg, whereas SAR-LDL lipids from these subjects contained 6, 9, 41, 46, and 7 nmol/mg. These results suggest that the different biological properties of SAR-LDL and SAP-LDL are related to their different carbohydrate compositions.

摘要

与正常受试者的低密度脂蛋白（LDL）导致人主动脉内皮下细胞胆固醇蓄积增加2%-17%相比，冠心病（CHD）患者的LDL可使胆固醇蓄积增加78%-286%。采用蓖麻毒蛋白-琼脂糖亲和色谱法将LDL分离为两个亚组分，一个富含唾液酸（SAR），另一个唾液酸含量低（SAP）。冠心病患者的SAP-LDL可使细胞胆固醇蓄积增加156%-307%，而这些患者的SAR-LDL仅使胆固醇蓄积增加14%-21%。正常健康受试者的SAP-LDL可使胆固醇蓄积增加50%-86%，而其SAR-LDL仅诱导增加2%-12%。对从4例冠心病患者分离的SAP-LDL蛋白进行碳水化合物分析，结果显示每毫克蛋白中N-乙酰葡糖胺、半乳糖、甘露糖和唾液酸的平均值分别为59、25、61和11纳摩尔。这些患者的SAR-LDL蛋白的平均值分别为59、31、77和24纳摩尔/毫克蛋白。对4例正常健康受试者的SAP-LDL蛋白分析表明，其平均值分别为58、29、72和22纳摩尔/毫克，而正常受试者的SAR-LDL蛋白含有59、29、72和29纳摩尔/毫克。LDL脂质中的碳水化合物含量约占脂蛋白中总碳水化合物的25%。4例冠心病患者的SAP-LDL脂质中，N-乙酰半乳糖胺、N-乙酰葡糖胺、半乳糖、葡萄糖和唾液酸的平均值分别约为2、2、18、18和2纳摩尔/毫克蛋白。这些患者的SAR-LDL脂质的平均值分别为3、4、34、41和5纳摩尔/毫克。对4例正常健康受试者的SAP-LDL脂质分析表明，其平均值分别为4、6、30、31和3纳摩尔/毫克，而这些受试者的SAR-LDL脂质含有6、9、41、46和7纳摩尔/毫克。这些结果表明，SAR-LDL和SAP-LDL不同的生物学特性与其不同的碳水化合物组成有关。