Wilson S J, Wilson H J
Department of Restorative Dentistry, Dental School, University of Birmingham, U.K.
J Oral Rehabil. 1993 Mar;20(2):225-31. doi: 10.1111/j.1365-2842.1993.tb01604.x.
A simple method of estimating traces of chlorhexidine in water using bromothymol blue is described. The procedure involves the extraction of the chlorhexidine/bromothymol blue complex into carbon tetrachloride from a buffered neutral solution and measurement of the absorbances of the extracts in a spectrophotometer. The extracted complex has good colour stability, and the method is accurate, reproducible, and very sensitive, with concentrations of chlorhexidine down to 0.05 micrograms ml-1 measurable.
本文描述了一种使用溴百里酚蓝估算水中洗必泰痕量的简单方法。该方法包括从缓冲中性溶液中将洗必泰/溴百里酚蓝络合物萃取到四氯化碳中，并在分光光度计中测量萃取液的吸光度。萃取得到的络合物具有良好的颜色稳定性，该方法准确、可重复且非常灵敏，可测量低至0.05微克/毫升的洗必泰浓度。
