Li Y, Bachmann S, Maiss E, Commandeur U, Breyel E, Timpe U, Koenig R
Institut für Biochemie und Pflanzenvirologie, Biologische Bundesanstalt für Land- und Forstwirtschaft, Braunschweig, Federal Republic of Germany.
Arch Virol. 1993;129(1-4):349-56. doi: 10.1007/BF01316911.
The sequence of 1,787 nucleotides (nts) in the genomic RNA of pelargonium leaf curl virus (PLCV) was determined. It included the entire coat protein (cp) gene (nts 585 to 1,754), 558 nts of the 3' end of the putative RNA polymerase gene, 26 nts of an intercistronic region between the two genes and 33 nts downstream of the stop codon of the cp gene. The cp gene was cloned into the expression vector pET8c and expressed in E. coli. The deduced cp amino acid sequence of PLCV was compared with those of five other tombusviruses. The closer the degree of serological relatedness between two viruses, the more similarity was found in their cp amino acid sequences not only in the protruding domains, but also in their random and shell domains and in the arm regions. Nucleic acid hybridization tests, cp amino acid comparisons and serological tests all suggest the same order of sequence for the relationships in the tombusvirus group.
测定了天竺葵叶卷曲病毒（PLCV）基因组RNA中1787个核苷酸（nts）的序列。它包括整个衣壳蛋白（cp）基因（nts 585至1754）、推定的RNA聚合酶基因3'端的558个nts、两个基因之间顺反子间区域的26个nts以及cp基因终止密码子下游的33个nts。将cp基因克隆到表达载体pET8c中并在大肠杆菌中表达。将PLCV推导的cp氨基酸序列与其他五种番茄病毒的序列进行了比较。两种病毒之间血清学相关性程度越接近，不仅在突出结构域，而且在其随机结构域、外壳结构域和臂区域的cp氨基酸序列中发现的相似性就越高。核酸杂交试验、cp氨基酸比较和血清学试验均表明番茄病毒组中关系的序列顺序相同。
