Wilson D, Paul P K, Roberts E D, Blancuzzi V, Gronlund-Jacob J, Vosbeck K, DiPasquale G, O'Byrne E M
Research Department, Ciba-Geigy Corp., Summit, New Jersey 07901.
Proc Soc Exp Biol Med. 1993 May;203(1):30-7. doi: 10.3181/00379727-203-43569.
Cartilage pathology in rabbit knees was monitored by noninvasive magnetic resonance imaging (MRI) and evaluated using morphometric histologic measurements. Infusion of rabbit knees with the cytokine interleukin 1 induces cartilage degradation and inflammation. A miniosmotic pump was implanted subcutaneously to deliver interleukin 1 through a polyethylene catheter inserted into the rabbit knee. Rabbit knees were imaged using MRI and prepared for histologic examination at 5 and 12 days after chronic infusion of interleukin 1. MRI obtained 0.7-mm sections for three-dimensional reconstruction of cartilage image. Cartilage deterioration near the site of infusion was visible on MRI. MRI measurements indicated a reduction in cartilage thickness. Histology revealed a loss of staining of cartilage matrix proteoglycan, synovial hypertrophy, and perichondral bone resorption. Morphometric analysis of cartilage histology indicated a reduction in both cellularity (chondrocytes/m mu 2 area) and cell to matrix area ratio. These observations suggest that a loss of proteoglycan, an early event in cartilage degeneration, can be detected by MRI.
通过无创磁共振成像（MRI）监测兔膝关节软骨病理情况，并使用形态计量组织学测量进行评估。向兔膝关节注入细胞因子白细胞介素1会导致软骨降解和炎症。皮下植入一个微型渗透泵，通过插入兔膝关节的聚乙烯导管输送白细胞介素1。在慢性注入白细胞介素1后的第5天和第12天，使用MRI对兔膝关节进行成像，并准备进行组织学检查。MRI获取0.7毫米厚的切片用于软骨图像的三维重建。在MRI上可见注入部位附近的软骨退化。MRI测量显示软骨厚度减小。组织学检查显示软骨基质蛋白聚糖染色缺失、滑膜肥大和软骨周骨吸收。软骨组织学的形态计量分析表明细胞密度（每平方毫米面积内的软骨细胞数量）和细胞与基质面积比均降低。这些观察结果表明，蛋白聚糖的丢失作为软骨退变的早期事件，可以通过MRI检测到。
