Hsiang Y N, Fragoso M, Tsang V, Schreiber W E
Department of Surgery, University of British Columbia, Vancouver, Canada.
Photochem Photobiol. 1993 Mar;57(3):518-25. doi: 10.1111/j.1751-1097.1993.tb02329.x.
The purpose of this study was to determine the lowest dose of Photofrin (P) that would produce a 3:1 or greater ratio between atherosclerotic (AS) and control arterial walls. Aortoiliac AS was created in 24 Yucatan miniswine by a combination of balloon endothelial injury and 2% cholesterol and 15% lard diet for 7 weeks. Arteriography was then performed to demonstrate AS lesions. Following this, swine were given intravenously P in one of the following single dosages: 2.5, 1.0 or 0.5 mg/kg. Twenty-four hours later, swine were sacrificed and aortoiliac and control carotid artery segments removed and photographed with ultraviolet light to differentiate fluorescent from nonfluorescent areas. Arterial specimens were submitted for histologic analysis and chemical extraction for determination of fluorescence using a spectrofluorometer. Tissue concentration (ng/g tissue) of P from AS vessels were: Group I, 130.4 +/- 82.7; Group II, 10.0 +/- 1.2; and Group III, 9.1 +/- 0.6, respectively (P < 0.05). Ratios of P concentration in AS: control vessels were: Group I, 8.1 +/- 13.7; Group II, 1.1 +/- 0.2; and Group III, 0.9 +/- 0.1, respectively (P < 0.05). These results demonstrated that a P dose of 2.5 mg/kg provided at least a 3:1 ratio between AS: control artery wall.
本研究的目的是确定能使动脉粥样硬化（AS）与对照动脉壁之间产生3:1或更高比例的光卟啉（P）的最低剂量。通过球囊内皮损伤、2%胆固醇和15%猪油饮食联合作用7周，在24只尤卡坦小型猪中诱发腹主动脉髂动脉AS。然后进行动脉造影以显示AS病变。在此之后，给猪静脉注射以下单一剂量之一的P：2.5、1.0或0.5mg/kg。24小时后，处死猪，取出腹主动脉髂动脉和对照颈动脉节段，用紫外光拍照以区分荧光区和非荧光区。将动脉标本送去进行组织学分析和化学提取，用荧光分光光度计测定荧光。AS血管中P的组织浓度（ng/g组织）分别为：第一组，130.4±82.7；第二组，10.0±1.2；第三组，9.1±0.6（P<0.05）。AS与对照血管中P浓度的比值分别为：第一组，8.1±13.7；第二组，1.1±0.2；第三组，0.9±0.1（P<0.05）。这些结果表明，2.5mg/kg的P剂量能使AS与对照动脉壁之间至少达到3:1的比例。
