Hsiang Y, Stonefield M, Bower R D, Fragoso M, Tsang V, Crespo M T, Lundkvist A
Department of Surgery, University of British Columbia, Vancouver, Canada.
Lasers Surg Med. 1993;13(3):271-8. doi: 10.1002/lsm.1900130303.
The purpose of this study was to assess Photofrin porfimer sodium (P*) concentration in atherosclerotic plaque (ASP) using a fluorescence detector (Fluoroprobe) compared with fluorescent photography and chemical extraction of P*. ASP was created in the aortoiliac segments of Yucatan miniswine by a combination of balloon endothelial injury and 2% cholesterol and 15% lard diet for 7 weeks. At that time, swine were given P* I.V. in one of the following single dosages: Group I, 2.5; Group II, 1.0; or Group III, 0.5 mg/kg. Swine were sacrificed 24 hours later and aortoiliac and control carotid artery segments removed. Fluorescence was determined from these segments using photographic techniques, the Fluoroprobe, and a spectrofluorometer after chemical extraction. ASP were identified in all swine using photography and the Fluoroprobe. The intensity of fluorescence measured with the Fluoroprobe for Groups I to III was 1,098 +/- 524, 471 +/- 337, and 295 +/- 173 units, respectively (P < 0.01). The tissue concentration of P* in ASP from each group was 130.4 +/- 82.7, 10.0 +/- 1.2, and 9.1 +/- 0.6 ng/g, respectively (P < 0.01). There was a linear correlation between the fluorescence intensity measured with the Fluoroprobe and the extracted tissue concentration (r = 0.88, P < 0.0001). This study showed that a fluorescent detector such as the Fluoroprobe accurately detects the uptake of P* into atherosclerotic plaque.
本研究的目的是使用荧光探测器（Fluoroprobe）评估动脉粥样硬化斑块（ASP）中的卟吩姆钠（P*）浓度，并与荧光摄影和P的化学提取法进行比较。通过球囊内皮损伤以及给予2%胆固醇和15%猪油饮食7周，在尤卡坦小型猪的主动脉髂动脉段形成ASP。此时，给猪静脉注射P，剂量如下：第一组，2.5mg/kg；第二组，1.0mg/kg；第三组，0.5mg/kg。24小时后处死猪，取出主动脉髂动脉段和对照颈动脉段。化学提取后，使用摄影技术、Fluoroprobe和荧光分光光度计测定这些段的荧光。使用摄影和Fluoroprobe在所有猪中均识别出ASP。第一组至第三组使用Fluoroprobe测量的荧光强度分别为1098±524、471±337和295±173单位（P<0.01）。每组ASP中P的组织浓度分别为130.4±82.7、10.0±1.2和9.1±0.6ng/g（P<0.01）。使用Fluoroprobe测量的荧光强度与提取的组织浓度之间存在线性相关性（r = 0.88，P<0.0001）。本研究表明，诸如Fluoroprobe的荧光探测器可准确检测P在动脉粥样硬化斑块中的摄取情况。
